Streptavidin 抗体
(3 references)Quick Overview for Streptavidin 抗体 (ABIN398439)
抗原
See all Streptavidin 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Streptavidin Antibody is specific to streptavidin.
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纯化方法
- Protein G chromatography
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免疫原
- Purified recombinant streptavidin
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亚型
- IgG
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anti-Streptavidin (AA 25-183) antibody
适用: Streptomyces avidinii ELISA, WB 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Streptavidin (C-Term) antibody适用: Streptomyces avidinii WB 宿主: 山羊 Polyclonal unconjugated
anti-Streptavidin (AA 25-183) antibody适用: Streptomyces avidinii ELISA, WB 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Streptavidin antibody (FITC)适用: Streptomyces avidinii FACS, IF 宿主: 兔 Polyclonal FITC
anti-Streptavidin antibody适用: Streptomyces avidinii ELISA, WB, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-Streptavidin antibody (Texas Red (TR))适用: Streptomyces avidinii FACS, FM, FLISA 宿主: 兔 Polyclonal Texas Red (TR)
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应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.5-1 µg/mL Dot blot: 0.5-1 µg/mL Western blot: 0.5-1 µg/mL Other applications: user-optimized -
限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- PBS, pH7.4, containing 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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储存条件
- 4 °C/-20 °C
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储存方法
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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: "Nutritional control of antibiotic resistance via an interface between the phosphotransferase system and a two-component signaling system." in: Antimicrobial agents and chemotherapy, Vol. 58, Issue 2, pp. 957-65, (2014) (PubMed).
: "Novel ubiquitin-derived high affinity binding proteins with tumor targeting properties." in: The Journal of biological chemistry, Vol. 289, Issue 12, pp. 8493-507, (2014) (PubMed).
: "Development of a novel multiplex lateral flow assay using an antimicrobial peptide for the detection of Shiga toxin-producing Escherichia coli." in: Journal of microbiological methods, Vol. 93, Issue 3, pp. 251-6, (2013) (PubMed).
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: "Nutritional control of antibiotic resistance via an interface between the phosphotransferase system and a two-component signaling system." in: Antimicrobial agents and chemotherapy, Vol. 58, Issue 2, pp. 957-65, (2014) (PubMed).
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- Streptavidin
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背景
- Streptavidin is a tetrameric protein from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of almost equal to10-14 mol/L. The high-affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Streptavidin Antibody is developed in rabbit using purified Streptavidin and highly purified from rabbit antiserum by protein G resin.
抗原
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