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Albumin 抗体 (HRP)

This 兔 多克隆 antibody specifically detects Albumin in WB, IF, EIA, ID, RIA 和 IHC (fro). It exhibits reactivity toward 小鼠.
产品编号 ABIN784423
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Albumin 抗体 (HRP) (ABIN784423)

抗原

See all Albumin (ALB) 抗体
Albumin (ALB)

适用

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小鼠

宿主

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克隆类型

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多克隆

标记

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This Albumin antibody is conjugated to HRP

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 特异性

    Tested in Immunoelectrophoresis and Double Radial Immunodiffusion against pooled normal Mouse serum and purified Mouse Albumin. One characteristic precipitin line is obtained against pooled normal Mouse serum using different antigen/antibody concentration ratio's. Precipitin lines against normal Mouse serum and purified Mouse Albumin give a reaction of full identity. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum has been tested for cross-reactivity in Double Radial Immunodiffusion with the following results: Bovine (-), Chicken (-), Canine (-), Goat (-), Guinea Pig (++), Hamster (+), Horse (+), Human (+), Monkey (+), Rat (++), Sheep (-), Swine (+). A negative cross-reaction in Double Radial Immunodiffusion does not exclude some reaction in more sensitive techniques.

    产品特性

    Molar Ratio: Enzyme/IgG ~1.7

    纯化方法

    Sequential Precipitation, Ion Exchange Chromatography and Affinity Chromatography

    免疫原

    Albumin is isolated from Mouse serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    亚型

    IgG
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 状态

    Liquid

    溶解方式

    Restore by adding 1 mL of sterile distilled water

    浓度

    10 mg/mL

    缓冲液

    PBS, pH 7.2 without preservatives and foreign proteins

    储存液

    Without preservative

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • 抗原

    Albumin (ALB)

    别名

    Albumin

    背景

    Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.Synonyms: ALB, BSA, HSA, Serum Albumin

    基因ID

    11657

    NCBI登录号

    NP_033784

    途径

    Lipid Metabolism
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