Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
特异性
Specific for the ~58 kDa Fto protein in Western blots of rat testes lysate. The antibody has also been used for Immunocytochemistry with neuronal progenitor cells.
交叉反应 (详细)
Species reactivity (expected):Bovine, Canine, Horse, non-Human Primate and Sheep. Species reactivity (tested):Human, Mouse, Rat.
纯化方法
Protein G Chromatography of Culture Supernatant
免疫原
Synthetic peptide -KLH conjugated corresponding to amino acid residues from the N- terminal region of FTO
The FTO gene is the most robust gene for common obesity characterized to date. FTO gene expression has been found to be significantly upregulated in the hypothalamus of rats after food deprivation and strongly negatively correlated with the expression of orexin peptide which is involved in the stimulation of food intake (Fredricksson R et al., 2008). Deletion analysis of FTO gene in mice showed that Fto is functionally involved in the control of both energy intake and energy expenditure (Fischer J et al., 2009).Synonyms: Fat mass and obesity-associated protein, KIAA1752