BrdU 抗体
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北京 101111
Quick Overview for BrdU 抗体 (ABIN782023)
抗原
See all BrdU 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein A Chromatography
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亚型
- IgG1
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL (OD280nm, E0.1% = 1.4)
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缓冲液
- 10 mM Tris, pH 7.5 containing 150 mM Sodium Chloride and 0.09 % Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- BrdU (Bromodeoxyuridine (BrdU))
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物质类
- Chemical
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背景
- Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells.
抗原
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