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PROL1 抗体 (Internal Region)

Verified PROL1 适用: 小鼠 WB, IHC, ELISA, IP, IF 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN768578
发货至: 中国
  • 抗原 See all PROL1 抗体
    PROL1 (Proline Rich, Lacrimal 1 (PROL1))
    抗原表位
    Internal Region
    适用
    • 3
    • 1
    • 1
    小鼠
    宿主
    • 3
    • 2
    山羊
    克隆类型
    • 3
    • 2
    多克隆
    标记
    • 5
    This PROL1 antibody is un-conjugated
    应用范围
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)
    原理
    Mucin 10 / Prol1
    序列
    QFPVRKYLED PRY
    交叉反应
    小鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-QFPVRKYLEDPRY, from the internal region of the protein sequence according to NP_032670.2.
    亚型
    IgG
  • 应用备注

    Immunohistochemistry: Positive staining in the submandibular salivary gland of the mouse, while cells remain negative in the sublingual salivary gland. Data provided by Everest Grant winner Melinda Larsen State University of New York, Albany, NY. A publi

    Western Blot: This antibody has been successfully used in WB on Mouse: Peluso G et al. (2019) J Biol Chem. 2019 Dec 27. pii: jbc.RA119.009807. PMID: 31882545.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: This antibody has been successfully used in IF on Mouse: Peluso G et al. (2019) J Biol Chem. 2019 Dec 27. pii: jbc.RA119.009807. PMID: 31882545.

    Immunoprecipitation: This antibody has been successfu

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Tanaka, Ogawa, Hojo, Kawashima, Mabuchi, Hata, Nakamura, Yasuhara, Takamatsu, Irié, Fukada, Sakai, Inoue, Nishimura, Ohara, Saito, Ohba, Tsuji, Mishima: "Generation of orthotopically functional salivary gland from embryonic stem cells." in: Nature communications, Vol. 9, Issue 1, pp. 4216, (2019) (PubMed).

    Nelson, Manhardt, Kamath, Sui, Santamaria-Pang, Can, Bello, Corwin, Dinn, Lazare, Gervais, Sequeira, Peters, Ginty, Gerdes, Larsen: "Quantitative single cell analysis of cell population dynamics during submandibular salivary gland development and differentiation." in: Biology open, Vol. 2, Issue 5, pp. 439-47, (2013) (PubMed).

  • 抗原
    PROL1 (Proline Rich, Lacrimal 1 (PROL1))
    别名
    Prol1 (PROL1 产品)
    别名
    BPLP antibody, PRL1 antibody, opiorphin antibody, Muc10 antibody, opiorphin prepropeptide antibody, proline rich, lacrimal 1 antibody, OPRPN antibody, Prol1 antibody
    背景
    Prol1, proline rich, lacrimal 1, Muc10, mucin 10, mucin 10, submandibular gland salivary mucin
    分子量
    45kDa
    基因ID
    17830
    NCBI登录号
    NP_032670
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