SIRT3 抗体 (Internal Region)
Quick Overview for SIRT3 抗体 (Internal Region) (ABIN768568)
抗原
See all SIRT3 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- Sirtuin 3 (mouse)
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序列
- RASGIPASKL VEAH
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特异性
- This antibody is expected to recognize both reported isoforms (NP_071878.2, NP_001171275.1). Reported variants represent identical proteins: NP_071878.2, NP_001120823.1
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交叉反应
- 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-RASGIPASKLVEAH, from the internal region of the protein sequence according to NP_071878.2, NP_001171275.1.
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亚型
- IgG
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anti-Sirtuin 3 (SIRT3) (C-Term) antibody
SIRT3 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Sirtuin 3 (SIRT3) (AA 250-279), (C-Term) antibodySIRT3 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB01967 unconjugated
anti-Sirtuin 3 (SIRT3) (AA 126-399) antibodySIRT3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sirtuin 3 (SIRT3) (AA 127-303) antibodySIRT3 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sirtuin 3 (SIRT3) (AA 155-290) antibodySIRT3 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 6B2A1 unconjugated
anti-Sirtuin 3 (SIRT3) (AA 304-334), (C-Term) antibodySIRT3 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB25945 unconjugated
anti-Sirtuin 3 (SIRT3) (AA 304-334) antibodySIRT3 适用: 人, 小鼠, Hamster WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Sirtuin 3 (SIRT3) (AA 250-279) antibodySIRT3 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Sirtuin 3 (SIRT3) (AA 5-257) antibodySIRT3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sirtuin 3 (SIRT3) (AA 20-130) antibodySIRT3 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: Approx 30 kDa band observed in Mouse Heart lysates (calculated MW of 28.8 kDa according to NP_071878.2). An additional band of unknown identity was also consistently observed at 15 kDa. This band was successfully blocked by incubation with the
Peptide ELISA: antibody detection limit dilution 1:64000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- SIRT3 (Sirtuin 3 (SIRT3))
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别名
- Sirt3
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背景
- Sirt3, sirtuin 3, sirtuin 3 (silent mating type information regulation 2, homolog) 3, 2310003L23Rik, AI848213, Sir2l3, NAD-dependent deacetylase sirtuin-3, OTTMUSP00000027441, OTTMUSP00000027442, OTTMUSP00000027444, OTTMUSP00000027445, SIR2-like protein 3
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分子量
- 28.8kDa according to NP_071878.2
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基因ID
- 23410, 64384, 293615
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NCBI登录号
- NP_071878, NP_001171275
抗原
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