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WT1 抗体

The 兔 单克隆 anti-WT1 antibody is suitable to detect WT1 in samples from 人, 大鼠 和 小鼠. It has been validated for IHC, WB, IF, ELISA 和 IP.
产品编号 ABIN8125749
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for WT1 抗体 (ABIN8125749)

抗原

See all WT1 抗体
WT1 (Wilms Tumor 1 (WT1))

适用

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人, 大鼠, 小鼠

宿主

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兔

克隆类型

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单克隆

标记

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This WT1 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP)

克隆位点

AB1838
  • 原理

    Wilms Tumor Protein Rabbit mAb

    特异性

    This antibody detects endogenous levels of Wilms Tumor Protein protein.

    纯化方法

    The antibody was purified by protein A affinity chromatography.

    免疫原

    Recombinant protein (or fragment).

    亚型

    IgG
  • 应用备注

    WB, 1:1000-1:5000 | IHC, 1:1000-1:5000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid freeze / thaw cycles

    储存条件

    -20 °C

    储存方法

    Store at-20°C. Avoid freeze / thaw cycles.
  • 抗原

    WT1 (Wilms Tumor 1 (WT1))

    别名

    Wilms Tumor Protein

    背景

    Synonyms: Wilms tumor protein (WT33)

    Background: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015],

    Gene Name: WT1

    Protein Name: Wilms Tumor Protein

    基因ID

    7490

    UniProt

    P19544

    途径

    Tube Formation
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