CEP55 抗体 (pSer425)
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北京 101111
Quick Overview for CEP55 抗体 (pSer425) (ABIN8120461)
抗原
See all CEP55 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer425
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原理
- CEP55 (phospho Ser425) Antibody
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特异性
- Phospho-CEP55 (S425) Polyclonal Antibody detects endogenous levels of CEP55 protein only when phosphorylated at S425.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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免疫原
- Synthesized phospho-peptide around the phosphorylation site of human CEP55 (phospho Ser425)
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亚型
- IgG
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anti-Centrosomal Protein 55kDa (CEP55) antibody
KD Validated CEP55 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB14080 unconjugated
anti-Centrosomal Protein 55kDa (CEP55) (AA 1-464) antibodyCEP55 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6A10 unconjugated
anti-Centrosomal Protein 55kDa (CEP55) (AA 151-250) antibodyCEP55 适用: 人 WB, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Centrosomal Protein 55kDa (CEP55) (AA 1-464) antibodyCEP55 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Centrosomal Protein 55kDa (CEP55) (AA 1-260) antibodyCEP55 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Centrosomal Protein 55kDa (CEP55) antibodyCEP55 适用: 人, 大鼠, 小鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Centrosomal Protein 55kDa (CEP55) (AA 1-464) antibodyCEP55 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Centrosomal Protein 55kDa (CEP55) (AA 38-64), (N-Term) antibodyCEP55 适用: 人 WB 宿主: 兔 Polyclonal RB22365 unconjugated
anti-Centrosomal Protein 55kDa (CEP55) (N-Term) antibodyCEP55 适用: 人, 犬, 豚鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Centrosomal Protein 55kDa (CEP55) (AA 360-409) antibodyCEP55 适用: 人, 大鼠, 小鼠, 犬, 豚鼠, 马, Cow, 兔, Pig, 猴, Bat WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C/1 year
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有效期
- 12 months
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- CEP55 (Centrosomal Protein 55kDa (CEP55))
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别名
- CEP55
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背景
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Synonyms: CEP55, C10orf3, URCC6, Centrosomal protein of 55 kDa, Cep55, Up-regulated in colon cancer 6
Background: function:Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.,PTM:There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.,subcellular location:Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loss of affinity for the centrosome at the onset of prophase and diffusion throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis.,subunit:Homodimer. Interacts (phosphorylated on Ser-425 and Ser-428) with PLK1. Interacts with AKAP9, the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PCNT, the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PDCD6IP, the interaction is direct, CEP55 binds PDCD6IP in a 2:1 stoechiometry, PDCD6IP competes with TSG101 for the same binding site. Interacts with TSG101, TSG101 competes with PDCD6IP for the same binding site, interaction is required for cytokinesis but not for viral budding. Interacts with FAM125A, VPS37B, VPS37C and VPS28.,tissue specificity:Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.,
Gene Name: CEP55
Protein Name: Centrosomal protein of 55 kDa
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基因ID
- 55165
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UniProt
- Q53EZ4
抗原
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