MYCN 抗体 (AA 401-464)
(1 reference)
(1 validation)Quick Overview for MYCN 抗体 (AA 401-464) (ABIN760676)
抗原
See all MYCN 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 401-464
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预测反应
- Rat,Cow,Pig
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated synthetic peptide derived from human n-Myc
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亚型
- IgG
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anti-N-Myc Proto-Oncogene Protein (MYCN) (AA 168-267) antibody
MYCN 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-N-Myc Proto-Oncogene Protein (MYCN) (AA 337-464) antibodyMYCN 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 2A4E9 unconjugated
anti-N-Myc Proto-Oncogene Protein (MYCN) (AA 1-100) antibodyMYCN 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 3H4 unconjugated
anti-N-Myc Proto-Oncogene Protein (MYCN) antibodyMYCN 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-N-Myc Proto-Oncogene Protein (MYCN) antibodyMYCN 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-N-Myc Proto-Oncogene Protein (MYCN) (AA 1-464) antibodyMYCN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-N-Myc Proto-Oncogene Protein (MYCN) (AA 1-100) antibodyMYCN 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4H4 unconjugated
anti-N-Myc Proto-Oncogene Protein (MYCN) (C-Term) antibodyMYCN 适用: 人 WB, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-N-Myc Proto-Oncogene Protein (MYCN) (Internal Region) antibodyMYCN 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-N-Myc Proto-Oncogene Protein (MYCN) (AA 322-351), (C-Term) antibodyMYCN 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB18771 unconjugated
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应用备注
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ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 -
限制
- 仅限研究用
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- by
- Alamo Laboratories Inc
- No.
- #029800
- 日期
- 2014.08.21
- 抗原
- Lot Number
- 120917
- Method validated
- Western Blotting
- Positive Control
- MCF7 cells (variant MCF7/VP)00284-0/pdf)
- Negative Control
- SK-N-SH cells - RNA
- Notes
- A strong band was observed in the positive control sample at the correct molecular weight. An additional band was also observed in the positive sample at a higher molecular weight which may represent non-specific binding. No bands were observed in the negative control sample.
- Primary Antibody
- Antigen: Mycn (MYCN)
- Catalog number: ABIN760676
- Lot number: 120917
- Antibody Dilution: 1:200
- Secondary Antibody
- Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
- Lot number: L170-6515
- Antibody Dilution: 1:10,000
- Full Protocol
- 1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
- 2. 15 μl of heated culture-media were loaded and resolved on 8-16% SDS-polyacrylamide gel.
- 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
- 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
- 5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
- 6. The membrane was rinsed with TBST once.
- 7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 13 hours at 4°C.
- 8. The membrane was rinsed in TBST thrice for 5 minutes each.
- 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
- 10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
- 11. The membrane was rinsed in TBS twice for 30 seconds each.
- 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 45 minutes.
- 13. The membrane was rinsed three times TBST.
- 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
- 15. The membrane was washed in TBST 2 times for 10 minutes each.
- 16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.
- Experimental Notes
- - No experimental challenges noted.
生效 #029800 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Figure 1: Western Blot for MYCN. Arrowhead indicates the expected molecular weight of ~49 kDa.]()
Figure 1: Western Blot for MYCN. Arrowhead indicates the expected molecular weight of ~49 kDa.
Full Methods -
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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有效期
- 12 months
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: "Serum-circulating miRNAs predict neuroblastoma progression in mouse model of high-risk metastatic disease." in: Oncotarget, Vol. 7, Issue 14, pp. 18605-19, (2016) (PubMed).
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: "Serum-circulating miRNAs predict neuroblastoma progression in mouse model of high-risk metastatic disease." in: Oncotarget, Vol. 7, Issue 14, pp. 18605-19, (2016) (PubMed).
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- MYCN (N-Myc Proto-Oncogene Protein (MYCN))
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别名
- N-Myc
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背景
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This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. [provided by RefSeq, Jul 2008].
Subcellular location: Nucleus
Synonyms: NMYC, ODED, MODED, N-myc, bHLHe37, N-myc proto-oncogene protein, Class E basic helix-loop-helix protein 37, MYCN
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基因ID
- 4613
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UniProt
- P04198
抗原
-
