Recombinant Villin 1 抗体 (AA 600-700)
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Quick Overview for Recombinant Villin 1 抗体 (AA 600-700) (ABIN8093561)
抗原
See all Villin 1 (VIL1) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 600-700
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原理
- Villin Antibody
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免疫原
- A recombinant fragment (around amino acids 600-700) of human Villin protein (exact sequence is proprietary) was used as the immunogen for the Villin Antibody for IHC / VIL1 Immunohistochemistry Antibody.
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亚型
- IgG, kappa
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anti-Villin 1 (VIL1) (AA 1-320) antibody
VIL1 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Villin 1 (VIL1) (AA 1-209) antibodyVIL1 适用: 人 IHC, WB, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 3E5G11 unconjugated
anti-Villin 1 (VIL1) (N-Term) antibodyVIL1 适用: 人 WB, IF, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Villin 1 (VIL1) (AA 180-207), (N-Term) antibodyVIL1 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB17824 unconjugated
anti-Villin 1 (VIL1) (AA 1-209) antibodyVIL1 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 5E3B2 unconjugated
anti-Villin 1 (VIL1) (C-Term) antibodyVIL1 适用: 人, 小鼠, 大鼠 IHC, WB, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Villin 1 (VIL1) (AA 179-311) antibodyVIL1 适用: 人 IHC, WB 宿主: 小鼠 Monoclonal VIL1-1314 unconjugated
anti-Villin 1 (VIL1) (AA 1-421) antibodyVIL1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Villin 1 (VIL1) (AA 179-311) antibodyVIL1 适用: 人 IHC, WB 宿主: 小鼠 Monoclonal rVIL1-1325 unconjugated Recombinant Antibody
anti-Villin 1 (VIL1) (AA 600-700) antibodyVIL1 适用: 人 IHC 宿主: 兔 Monoclonal 459R unconjugated Recombinant Antibody
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应用备注
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1. Optimal dilution of the Villin Antibody for IHC / VIL1 Immunohistochemistry Antibody should be determined by the researcher.
2. This VIL1/Villin antibody is recombinantly produced by expression in human HEK293 cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- VIL1/Villin antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.
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- Villin 1 (VIL1)
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别名
- Villin
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背景
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Villin-1 (VIL1) is an actin-associated protein localized to the apical region of intestinal epithelial cells, where it supports brush border organization and epithelial architecture. Villin Antibody for IHC / VIL1 Immunohistochemistry Antibody (clone MSVA-459R) is specifically developed for immunohistochemistry applications, enabling consistent detection of Villin in formalin-fixed, paraffin-embedded (FFPE) tissue sections. Villin antibody, also known as Villin-1 antibody or VIL1 antibody, is widely used in histological analysis to visualize epithelial structures and glandular morphology in gastrointestinal tissues.
This Villin Antibody for IHC is strongly differentiated by its focus on immunohistochemistry performance, FFPE compatibility, and large-scale tissue validation rather than purely biological function. The recombinant rabbit monoclonal format (clone MSVA-459R) supports high signal clarity and reproducibility, which is critical for IHC workflows. Importantly, this antibody has been evaluated using human tissue microarray (TMA) panels, demonstrating consistent staining across a broad range of normal and cancer tissues. This TMA-based validation provides confidence in staining specificity and pattern consistency in complex tissue environments.
In immunohistochemistry, Villin produces a characteristic apical and cytoplasmic staining pattern in epithelial cells, clearly outlining luminal borders and glandular structures. This makes Villin Antibody for IHC particularly valuable for assessing epithelial organization, identifying gland-forming tissues, and visualizing enterocyte-like differentiation in tumors. In colorectal and other gastrointestinal adenocarcinomas, Villin staining highlights tumor epithelial cells and supports evaluation of epithelial morphology within FFPE sections.
From a pathology and research perspective, this VIL1 Immunohistochemistry Antibody is optimized for tissue-based analysis, including FFPE sections and TMA studies, where reproducible staining and clear morphological definition are essential. Clone MSVA-459R provides a robust tool for investigators and pathologists studying epithelial architecture, tumor histology, and gastrointestinal tissue identity using immunohistochemistry.
This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues. -
UniProt
- P09327
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途径
- EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
抗原
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