Recombinant S100A12 抗体 (AA 1-95)
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Quick Overview for Recombinant S100A12 抗体 (AA 1-95) (ABIN8093448)
抗原
See all S100A12 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-95
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原理
- S100A12 Antibody / Calgranulin C / EN-RAGE
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免疫原
- A recombinant fragment (around amino acids 1-95) of human S100A12 protein (exact sequence is proprietary) was used as the immunogen for the S100A12/S100 calcium binding protein A12 antibody.
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亚型
- IgG1, kappa
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anti-S100 Calcium Binding Protein A12 (S100A12) (AA 2-92) antibody
S100A12 适用: Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A12 (S100A12) (AA 2-92) antibodyS100A12 适用: Cow WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A12 (S100A12) (AA 1-92) antibodyS100A12 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A12 (S100A12) antibodyS100A12 适用: 人 IHC 宿主: 小鼠 Monoclonal 812M unconjugated
anti-S100 Calcium Binding Protein A12 (S100A12) (AA 1-92) antibodyS100A12 适用: 人 WB 宿主: 小鼠 Monoclonal 15# unconjugated
anti-S100 Calcium Binding Protein A12 (S100A12) (AA 2-91) antibodyS100A12 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A12 (S100A12) antibodyS100A12 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A12 (S100A12) (AA 1-92) antibodyS100A12 适用: 人 WB 宿主: 小鼠 Monoclonal 1F10 unconjugated
anti-S100 Calcium Binding Protein A12 (S100A12) antibodyS100A12 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A12 (S100A12) (Internal Region) antibodyS100A12 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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1. Optimal dilution of the S100A12 Antibody for IHC / S100 Calcium Binding Protein A12 Immunohistochemistry Antibody should be determined by the researcher.
2. This S100A12/S100 calcium binding protein A12 antibody is recombinantly produced by expression in CHO cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- S100A12/S100 calcium binding protein A12 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.
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- S100A12 (S100 Calcium Binding Protein A12 (S100A12))
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别名
- S100A12
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背景
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S100 calcium binding protein A12 (S100A12) is a calcium-binding protein encoded by the S100A12 gene and belonging to the S100 family of EF-hand calcium-binding proteins. S100A12 Antibody for IHC enables visualization of S100 Calcium Binding Protein A12 expression in formalin-fixed paraffin-embedded tissue sections using immunohistochemistry. S100A12, also referred to as Calgranulin C or EN-RAGE (extracellular newly identified RAGE-binding protein), is predominantly expressed by neutrophils and activated myeloid cells where it functions as a pro-inflammatory mediator involved in innate immune responses and inflammatory signaling pathways.
Immunohistochemistry studies commonly detect S100A12 protein within neutrophils, granulocytes, and other inflammatory cell populations present in tissues undergoing inflammatory responses. In histological sections, S100A12 staining typically appears within the cytoplasm of infiltrating neutrophils and inflammatory cells, allowing clear visualization of these cells within the surrounding tissue architecture. Because S100A12 is associated with inflammatory cell infiltration, immunohistochemical staining is often used to evaluate inflammatory microenvironments and immune cell presence within tissue samples.
Large-scale tissue expression analysis using human tissue microarray (TMA) panels provides an efficient method for examining S100A12 distribution across many tissue types simultaneously. In human tissue microarray studies containing multiple normal and cancer tissues, S100A12 staining is generally restricted to scattered inflammatory cells rather than epithelial or stromal compartments. These patterns allow investigators to distinguish inflammatory cell populations within different tissues and to evaluate immune infiltration across a broad spectrum of tissue environments while maintaining consistent staining conditions across all specimens.
A recombinant mouse monoclonal antibody, clone MSVA-812M, recognizes S100A12 protein and supports immunohistochemical detection of S100A12 in FFPE human tissues. When applied to human tissue microarray (TMA) panels containing multiple normal and cancer tissues, S100A12 Antibody for IHC highlights neutrophils and other myeloid-derived inflammatory cells present within tissue sections. These staining patterns allow researchers to visualize inflammatory cell localization directly within histological tissue architecture and facilitate studies examining immune cell distribution and inflammatory responses in normal and disease-associated tissues.
This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues. -
UniProt
- P80511
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途径
- Toll-Like Receptors Cascades, S100 Proteins
抗原
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