Recombinant LIPE 抗体 (pSer853)
Our Local Distributor
北京 101111
Quick Overview for Recombinant LIPE 抗体 (pSer853) (ABIN8093300)
抗原
See all LIPE 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- pSer853
-
原理
- Phospho-LIPE (pSer853) Antibody / Hormone-sensitive lipase / HSL
-
纯化方法
- Affinity chromatography
-
免疫原
- A synthesized peptide derived from human Phospho-Hormone-sensitive lipase (pS853) was used as the immunogen for the Phospho-LIPE (pSer853) antibody.
-
亚型
- IgG
-
-
anti-Lipase, Hormone-Sensitive (LIPE) (AA 822-849), (C-Term) antibody
LIPE 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB17399 unconjugated
anti-Lipase, Hormone-Sensitive (LIPE) (pSer863) antibodyLIPE 适用: 大鼠 WB, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lipase, Hormone-Sensitive (LIPE) (pSer554), (pSer855) antibodyLIPE 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lipase, Hormone-Sensitive (LIPE) (pSer552) antibodyLIPE 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lipase, Hormone-Sensitive (LIPE) (pSer563), (pSer853) antibodyLIPE 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lipase, Hormone-Sensitive (LIPE) (AA 1-251) antibodyLIPE 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Lipase, Hormone-Sensitive (LIPE) antibodyLIPE 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Lipase, Hormone-Sensitive (LIPE) (AA 900-1000) antibodyLIPE 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Lipase, Hormone-Sensitive (LIPE) (pSer552) antibodyLIPE 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Lipase, Hormone-Sensitive (LIPE) (pSer855) antibodyLIPE 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Optimal dilution of the Phospho-LIPE (pSer853) antibody should be determined by the researcher.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Store the Phospho-LIPE antibody at -20oC.
-
-
- LIPE (Lipase, Hormone-Sensitive (LIPE))
-
别名
- LIPE
-
背景
-
Phospho-LIPE (pSer853) antibody targets the phosphorylated form of Hormone-sensitive lipase (LIPE), also known as HSL, at serine 853, a well-characterized regulatory phosphorylation site associated with activation of lipolytic function. LIPE is a cytoplasmic neutral lipase that plays a central role in lipid mobilization by hydrolyzing triglycerides, diacylglycerols, and cholesteryl esters stored within lipid droplets. In metabolic cells, LIPE localizes primarily to the cytoplasm and lipid droplet surface, with phosphorylation-dependent activation in response to hormonal and signaling cues. Detection of phospho-LIPE provides insight into the activated, signal-responsive pool of HSL rather than total protein abundance.
Functionally, phosphorylation of LIPE at serine 853 is linked to kinase-driven signaling pathways that regulate lipolysis during periods of increased energy demand. This modification promotes enzymatic activation and facilitates interaction with lipid droplets, enabling efficient release of free fatty acids. Hormonal stimulation, such as catecholamine signaling, enhances LIPE phosphorylation, while opposing pathways suppress phosphorylation and favor lipid storage. A Phospho-LIPE (pSer853) antibody supports studies examining dynamic regulation of lipolysis and metabolic signaling pathways.
Phosphorylated LIPE plays an important role in adipocytes, skeletal muscle, and steroidogenic tissues, where controlled lipid mobilization is essential for energy production and biosynthetic processes. In adipose tissue, LIPE phosphorylation coordinates lipid breakdown during fasting or stress, while in muscle it contributes to fuel availability during energy demand. Monitoring phospho-LIPE levels enables assessment of pathway activation states rather than total enzyme expression, providing a functional readout of lipid metabolic regulation.
From a biological and disease-relevance perspective, dysregulation of LIPE phosphorylation is associated with altered lipid handling, insulin resistance, and metabolic disease. Aberrant signaling that affects LIPE activation can lead to impaired fatty acid release or excessive lipid accumulation. Phospho-LIPE therefore represents a valuable marker for studying signaling pathways that control lipid metabolism, metabolic flexibility, and hormone-responsive energy balance.
At the molecular level, LIPE is encoded by the LIPE gene and produces a cytoplasmic enzyme that migrates at approximately 80-90 kDa on SDS-PAGE under reducing conditions. Phosphorylation-dependent regulation of LIPE occurs in response to cellular metabolic state and upstream kinase activity. A Phospho-LIPE (pSer853) antibody supports research applications focused on lipid signaling, metabolic regulation, and post-translational control of hormone-sensitive lipase, with NSJ Bioreagents providing reagents intended for research use. -
UniProt
- Q05469
-
途径
- AMPK Signaling, Monocarboxylic Acid Catabolic Process, Lipid Metabolism
抗原
-
