Recombinant GLN1 抗体 (AA 100-200)
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Quick Overview for Recombinant GLN1 抗体 (AA 100-200) (ABIN8093214)
抗原
See all GLN1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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抗原表位
- AA 100-200
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原理
- Glutamine Synthetase Antibody / GLUL (azide and preservative free)
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免疫原
- A recombinant fragment (around amino acids 100-200) of human GLUL protein (exact sequence is proprietary) was used as the immunogen for the GLUL/Glutamine synthetase antibody.
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亚型
- IgG, kappa
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anti-Glutamine Synthetase (GLN1) (AA 1-373) antibody
GLN1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) (AA 1-373) antibodyGLN1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) (AA 285-373) antibodyGLN1 适用: 人 WB, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) (C-Term) antibodyGLN1 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) (AA 50-250) antibodyGLN1 适用: 人 IHC 宿主: 小鼠 Monoclonal 750M unconjugated
anti-Glutamine Synthetase (GLN1) (AA 1-373) antibodyGLN1 适用: 人 WB, ICC, IF, ELISA 宿主: 小鼠 Monoclonal AT8D7 unconjugated
anti-Glutamine Synthetase (GLN1) (AA 1-373) antibodyGLN1 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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1. Optimal dilution of the GLUL/Glutamine synthetase antibody should be determined by the researcher.
2. This GLUL/Glutamine synthetase antibody is recombinantly produced by expression in CHO cells. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- GLUL/Glutamine synthetase antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.
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- GLN1 (Glutamine Synthetase (GLN1))
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别名
- Glutamine Synthetase
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背景
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Glutamate-ammonia ligase is a cytosolic enzyme encoded by the GLUL gene and commonly referred to as Glutamine synthetase. GLUL Antibody for WB GLUL/12902R is developed for western blot detection of this key metabolic enzyme in protein lysates. GLUL catalyzes the ATP-dependent conversion of glutamate and ammonia into glutamine and plays an essential role in nitrogen metabolism, neurotransmitter recycling, and cellular metabolic regulation.
In western blot experiments, glutamine synthetase typically appears as a band near its predicted molecular weight of approximately 42 kDa on SDS-PAGE. Brain and liver lysates frequently produce strong immunoreactive signal because GLUL expression is enriched in astrocytes of the central nervous system and in pericentral hepatocytes within the liver. These tissues are therefore commonly used as positive controls in immunoblot workflows evaluating glutamine synthetase expression. Other tissues and cultured cell lines may show weaker bands depending on metabolic activity and cellular dependence on glutamine synthesis.
Western blot analysis is particularly useful for evaluating relative GLUL protein levels across tissues or experimental treatments. Because glutamine synthetase is a soluble cytoplasmic enzyme, it is typically extracted efficiently using standard detergent-based lysis buffers such as RIPA or NP-40. After electrophoresis and membrane transfer, GLUL most often appears as a single predominant immunoreactive band corresponding to the full-length protein, allowing clear interpretation of immunoblot results. In some samples, minor band variation may occur due to protein turnover products or post-translational modifications, but the dominant species generally aligns with the expected molecular size.
Immunoblot-based detection of GLUL is frequently used in studies examining metabolic regulation, hypoxia responses, and tumor-associated metabolic reprogramming. Altered glutamine synthetase expression has been described in hepatocellular carcinoma and other malignancies, where western blot analysis can help evaluate metabolic pathway activation and glutamine dependence. As a recombinant rabbit monoclonal reagent, GLUL Antibody for WB GLUL/12902R provides consistent target recognition suitable for immunoblot analysis of glutamine synthetase protein expression across diverse experimental systems. -
UniProt
- P15104
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途径
- Positive Regulation of Peptide Hormone Secretion
抗原
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