Recombinant Elastin 抗体 (full length)
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Quick Overview for Recombinant Elastin 抗体 (full length) (ABIN8093148)
抗原
See all Elastin (ELN) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- full length
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原理
- Elastin Antibody for IHC
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免疫原
- Recombinant full-length human Elastin protein was used as the immunogen for the ELN/Elastin antibody.
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亚型
- IgG, kappa
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anti-Elastin (ELN) (AA 681-786) antibody
ELN 适用: 人 WB, IF, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Elastin (ELN) (AA 392-645) antibodyELN 适用: 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Elastin (ELN) antibodyELN 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal ELN-1981 unconjugated
anti-Elastin (ELN) antibodyELN 适用: 人 IHC 宿主: 兔 Monoclonal ELN-3131R unconjugated Recombinant Antibody
anti-Elastin (ELN) antibodyELN 适用: 人 IHC, ELISA, IF, IHC (p), RIA 宿主: 兔 Polyclonal unconjugated
anti-Elastin (ELN) (full length) antibodyELN 适用: 人 IHC 宿主: 兔 Monoclonal 648R unconjugated Recombinant Antibody
anti-Elastin (ELN) (AA 266-443) antibodyELN 适用: 小鼠 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Elastin (ELN) (AA 392-645) antibodyELN 适用: 人 WB 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Elastin (ELN) antibodyELN 适用: 人, 小鼠, Cow, 小鸡 WB, IHC, IP, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 10B8 unconjugated
anti-Elastin (ELN) (AA 27-200) antibodyELN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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1. Optimal dilution of the Elastin Antibody for IHC / ELN Immunohistochemistry Antibody should be determined by the researcher.
2. This ELN/Elastin antibody is recombinantly produced by expression in human HEK293 cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- ELN/Elastin antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.
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- Elastin (ELN)
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别名
- Elastin
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背景
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Elastin (ELN) is a major extracellular matrix protein responsible for imparting elasticity and recoil to tissues such as arteries, lung, and skin. As the core structural component of elastic fibers, Elastin forms highly organized extracellular networks that enable tissues to withstand repeated mechanical stress. These fibers are particularly abundant in vascular walls, pulmonary structures, and dermal connective tissue, where they contribute to tissue compliance and integrity. In immunohistochemistry, Elastin is visualized as extracellular, fibrillar or lamellar HRP-DAB brown staining, providing a distinct pattern that outlines connective tissue architecture. Elastin Antibody for IHC is widely used in formalin-fixed, paraffin-embedded tissues to evaluate elastic fiber distribution and extracellular matrix organization in histological sections.
Elastin antibody, also referred to as ELN antibody or elastic fiber antibody in the literature, recognizes an extracellular matrix protein with highly specific localization to elastic fiber networks. This Elastin Antibody for IHC is specifically optimized for Tissue Microarray (TMA)-based immunohistochemistry, enabling standardized, high-throughput comparison of elastin distribution across large panels of normal and cancer tissues. In normal tissue TMAs, strong and well-defined extracellular HRP-DAB brown staining is consistently observed in elastic fiber-rich structures including arterial media, dermal connective tissue, and lung parenchyma, where elastic fibers form organized networks. In contrast, epithelial cells, lymphoid tissues, and most non-connective cellular compartments remain largely negative, producing a clean background that enhances interpretation of extracellular matrix patterns.
In cancer tissue microarrays, Elastin staining highlights stromal organization and extracellular matrix remodeling rather than direct tumor cell labeling. Elastic fibers are often preserved, fragmented, or redistributed within the tumor microenvironment, with staining outlining vascular structures, fibrous stroma, and connective tissue boundaries surrounding tumor cells. These patterns provide insight into tumor-associated extracellular matrix remodeling and vascular architecture, making Elastin Antibody for IHC a valuable tool for studying stromal composition and structural changes in cancer tissues.
Tissue Microarray (TMA) analysis enables side-by-side evaluation of elastin distribution across hundreds of tissue cores under identical staining conditions, demonstrating highly reproducible extracellular staining patterns in elastic fiber-rich tissues alongside minimal background in non-expressing regions. The performance of clone MSVA-648R in TMA-based IHC highlights its ability to generate crisp, well-defined visualization of elastic fiber networks across diverse tissue types. The observed staining patterns are consistent with established elastin biology and reference datasets such as the Human Protein Atlas, supporting confidence in its specificity and utility for large-scale tissue profiling studies.
This antibody targets Elastin in research applications requiring precise and interpretable immunohistochemical detection of extracellular matrix structures in FFPE tissue sections, making it well suited for studies of vascular biology, tissue elasticity, stromal architecture, and extracellular matrix remodeling.
This antibody is part of the < a href=../elastin-antibody.html>Elastin antibody collection, where additional ELN antibodies can be explored.
This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues. -
UniProt
- P15502
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途径
- Regulation of Actin Filament Polymerization
抗原
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