Recombinant EEA1 抗体
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北京 101111
Quick Overview for Recombinant EEA1 抗体 (ABIN8093137)
抗原
See all EEA1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- EEA1 Antibody / Early endosome antigen 1
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纯化方法
- Affinity chromatography
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免疫原
- A synthesized peptide derived from human Early endosome antigen 1 protein was used as the immunogen for the EEA1 antibody.
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亚型
- IgG
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anti-Early Endosome Antigen 1 (EEA1) (AA 1312-1411) antibody
EEA1 适用: 人 WB 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Early Endosome Antigen 1 (EEA1) (AA 1182-1411) antibodyEEA1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) antibodyKD Validated EEA1 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 23GB5260 unconjugated Recombinant Antibody
anti-Early Endosome Antigen 1 (EEA1) (AA 1312-1411) antibodyEEA1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Early Endosome Antigen 1 (EEA1) antibodyEEA1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) (AA 734-875) antibodyEEA1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) (C-Term) antibodyEEA1 适用: 人, 小鼠, 大鼠, 犬, 马, 兔, 猴, Pig, Cow, 豚鼠, 小鸡, Avian, Cat, 驴, 山羊, Hamster, 绵羊 WB, IF, IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) (AA 1312-1411) antibodyEEA1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) (C-Term) antibodyEEA1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) (AA 1251-1350) antibodyEEA1 适用: 人 IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the EEA1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the EEA1 antibody at -20oC.
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- EEA1 (Early Endosome Antigen 1 (EEA1))
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别名
- EEA1
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背景
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EEA1 antibody targets Early endosome antigen 1 (EEA1), a cytoplasmic protein that serves as a key marker and functional regulator of early endosomes within the endocytic pathway. EEA1 localizes predominantly to early endosomal membranes, where it participates in vesicle tethering and fusion events that govern intracellular trafficking. The protein contains characteristic domains, including a FYVE zinc finger domain that binds phosphatidylinositol 3-phosphate, enabling selective association with early endosome membranes enriched in this lipid. Through this localization, EEA1 helps define early endosomal identity and organization.
Functionally, EEA1 plays a central role in coordinating endocytic vesicle docking and homotypic fusion of early endosomes. By interacting with Rab5 and phosphoinositide lipids, EEA1 acts as a molecular tether that brings vesicles into proximity prior to membrane fusion. This process is essential for efficient sorting of internalized receptors, ligands, and membrane components. Proper EEA1 function ensures that cargo entering the cell through endocytosis is accurately directed toward recycling pathways or downstream trafficking compartments. An EEA1 antibody supports studies examining membrane trafficking and endosomal dynamics.
EEA1 expression is broadly observed across many cell types, reflecting the universal requirement for endocytosis in cellular homeostasis. The punctate cytoplasmic staining pattern associated with EEA1 corresponds to early endosomal structures and is frequently used as a reference marker in imaging studies. Analysis of EEA1 distribution and abundance provides insight into changes in endosomal organization that occur during receptor activation, signaling events, or cellular stress. Because early endosomes function as important signaling hubs, EEA1 is also relevant in studies linking trafficking to signal transduction.
From a biological and disease-relevance perspective, alterations in endocytic trafficking have been implicated in cancer, neurodegenerative disorders, and infectious disease processes. Dysregulation of early endosome function can impact receptor signaling duration, nutrient uptake, and pathogen entry. EEA1 is therefore commonly examined in studies investigating defects in intracellular transport, endosomal maturation, and membrane organization under pathological conditions. Monitoring EEA1 helps clarify how disruptions in early endosome biology contribute to disease-associated cellular phenotypes.
At the molecular level, EEA1 is encoded by the EEA1 gene and produces a large coiled-coil protein of approximately 180 kDa. The protein architecture supports long-range tethering interactions between membranes and recruitment of additional trafficking factors. EEA1 activity is regulated by lipid composition, small GTPase interactions, and cellular signaling state. An EEA1 antibody supports research applications focused on endocytosis, vesicle trafficking, and intracellular membrane dynamics, with NSJ Bioreagents providing reagents intended for research use. -
UniProt
- Q15075
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途径
- Toll-Like Receptors Cascades
抗原
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