Recombinant Desmoglein 1 抗体
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Quick Overview for Recombinant Desmoglein 1 抗体 (ABIN8093131)
抗原
See all Desmoglein 1 (DSG1) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- DSG1 Antibody / Desmoglein 1
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免疫原
- Bovine desmoglein (desmosomal glyco protein I) from nasal epidermis was used as the immunogen for the DSG1 antibody for IHC.
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亚型
- IgG2a, kappa
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anti-Desmoglein 1 (DSG1) antibody
DSG1 适用: 人 IHC 宿主: 小鼠 Monoclonal 32-2B unconjugated
anti-Desmoglein 1 (DSG1) antibodyDSG1 适用: 人 IHC 宿主: 小鼠 Monoclonal 544M unconjugated
anti-Desmoglein 1 (DSG1) antibodyDSG1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 1 (DSG1) (AA 800-1049) antibodyDSG1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 1 (DSG1) (AA 384-539) antibodyDSG1 适用: 人 IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 1 (DSG1) (AA 1-1049) antibodyDSG1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Desmoglein 1 (DSG1) (AA 1-1049) antibodyDSG1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 1 (DSG1) (Internal Region) antibodyVerified DSG1 适用: 人 IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Desmoglein 1 (DSG1) (Intracellular) antibodyDSG1 适用: 人 WB, IF, FACS 宿主: 小鼠 Monoclonal 18D4 unconjugated
anti-Desmoglein 1 (DSG1) (Intracellular) antibodyDSG1 适用: 人 IHC 宿主: 小鼠 Monoclonal DSG1-1733 unconjugated
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应用备注
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1. Optimal dilution of the DSG1 Antibody for IHC / Desmoglein 1 Antibody should be determined by the researcher.
2. This DSG1 / Desmoglein 1 is recombinantly produced by expression in CHO cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- DSG1 / Desmoglein 1 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.
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- Desmoglein 1 (DSG1)
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别名
- DSG1
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背景
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DSG1 Antibody for IHC detects Desmoglein 1 (DSG1), a calcium-dependent desmosomal cadherin encoded by the DSG1 gene on chromosome 18q12.1. Desmoglein 1 is a single-pass transmembrane glycoprotein localized to desmosomes at the plasma membrane of stratified epithelial cells, where it mediates strong intercellular adhesion and preserves epidermal structural integrity. A DSG1 Antibody for IHC is commonly used to evaluate epithelial differentiation, barrier formation, and desmosomal integrity in tissue sections. This mouse monoclonal antibody clone MSVA-544M supports immunohistochemical detection of membranous DSG1 expression in formalin-fixed tissues.
DSG1 antibody, also referred to as Desmoglein 1 antibody in the literature, recognizes a core structural component of desmosomal junctions within the cadherin superfamily. Its extracellular cadherin repeats mediate calcium-dependent homophilic adhesion between adjacent keratinocytes, while its cytoplasmic domain associates with desmosomal plaque proteins including Plakoglobin and Desmoplakin. Through these interactions, Desmoglein 1 anchors keratin intermediate filaments to the plasma membrane, reinforcing mechanical resilience in tissues exposed to friction and physical stress, particularly the epidermis and mucosal epithelium.
Expression of DSG1 is predominantly observed in the suprabasal layers of stratified squamous epithelia, including skin, oral mucosa, and esophagus, where it is enriched in differentiated keratinocytes and contributes to epidermal stratification and barrier function. A DSG1 Antibody for IHC typically demonstrates strong membranous staining in suprabasal epithelial layers and is frequently used in studies of squamous differentiation and epithelial organization. Autoantibodies directed against Desmoglein 1 are implicated in pemphigus foliaceus, where disruption of desmosomal adhesion results in superficial epidermal blistering. Altered DSG1 expression has also been associated with inherited skin disorders and inflammatory conditions affecting epithelial cohesion.
Structurally, Desmoglein 1 contains multiple extracellular cadherin domains, a transmembrane region, and an intracellular tail that connects to the desmosomal plaque complex. Beyond its structural role, Desmoglein 1 participates in signaling pathways that regulate keratinocyte differentiation and epidermal homeostasis. Dysregulated DSG1 expression may alter epithelial architecture and contribute to inflammatory or neoplastic processes in stratified epithelia. Through its essential role in maintaining desmosomal integrity, Desmoglein 1 remains central to research in epithelial biology, dermatologic disease mechanisms, and tumor-associated epithelial differentiation.
This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues. -
UniProt
- Q02413
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途径
- Cell-Cell Junction Organization
抗原
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