Recombinant CSTA 抗体 (full length)
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Quick Overview for Recombinant CSTA 抗体 (full length) (ABIN8093116)
抗原
See all CSTA 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- full length
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原理
- Cystatin A Antibody for IHC / CSTA
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免疫原
- Recombinant full-length human CSTA protein was used as the immuongen for the CSTA/Cystatin A antibody.
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亚型
- IgG2c, kappa
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anti-Cystatin A (Stefin A) (CSTA) antibody
CSTA 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal CPTC-CSTA-1 unconjugated
anti-Cystatin A (Stefin A) (CSTA) (AA 2-98) antibodyCSTA 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cystatin A (Stefin A) (CSTA) (AA 1-98) antibodyCSTA 适用: 人 IHC 宿主: 小鼠 Monoclonal CSTA-2882 unconjugated
anti-Cystatin A (Stefin A) (CSTA) (C-Term) antibodyCSTA 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cystatin A (Stefin A) (CSTA) (full length) antibodyCSTA 适用: 人 IHC 宿主: 小鼠 Monoclonal 461M unconjugated
anti-Cystatin A (Stefin A) (CSTA) (AA 1-98) antibodyCSTA 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cystatin A (Stefin A) (CSTA) (C-Term) antibodyCSTA 适用: 人, 小鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cystatin A (Stefin A) (CSTA) (AA 1-98) antibodyCSTA 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Cystatin A (Stefin A) (CSTA) antibodyCSTA 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB5665 unconjugated Recombinant Antibody
anti-Cystatin A (Stefin A) (CSTA) (AA 1-98) antibodyCSTA 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1A11 unconjugated
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应用备注
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1. Optimal dilution of the Cystatin A Antibody for IHC / CSTA Immunohistochemistry Antibody should be determined by the researcher.
2. This CSTA/Cystatin A antibody is recombinantly produced by expression in CHO cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- CSTA/Cystatin A antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.
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- CSTA (Cystatin A (Stefin A) (CSTA))
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别名
- Cystatin A
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背景
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Cystatin A (CSTA), also known as Stefin A, is a member of the cystatin family of cysteine protease inhibitors and is predominantly expressed in stratified squamous epithelial tissues. It plays an important role in regulating intracellular protease activity and maintaining epithelial barrier integrity during differentiation. In immunohistochemistry, Cystatin A is characteristically detected as cytoplasmic staining within squamous epithelial cells, making it a useful marker of epithelial differentiation. Cystatin A Antibody for IHC is widely used in formalin-fixed, paraffin-embedded tissues to visualize squamous cell populations and assess epithelial lineage in histological sections.
Cystatin A antibody, also referred to as CSTA antibody or Stefin A antibody in the literature, recognizes a cytoplasmic protein with strong and selective expression in squamous epithelia. This Cystatin A Antibody for IHC is specifically optimized for Tissue Microarray (TMA)-based immunohistochemistry, enabling high-throughput assessment of staining patterns across large panels of normal and cancer tissues under standardized conditions. In normal tissue TMAs, strong cytoplasmic HRP-DAB brown staining is consistently observed in stratified squamous epithelia such as skin, esophagus, and tonsil, while glandular tissues, stromal compartments, and non-epithelial cell populations remain largely negative, providing a clear contrast for interpretation.
In cancer tissue microarrays, Cystatin A expression is prominently detected in squamous cell carcinomas, where diffuse and intense cytoplasmic staining highlights tumor cells with squamous differentiation. This staining pattern is particularly evident in tumors of the head and neck, lung, and anogenital regions, where squamous lineage is a defining feature. In contrast, adenocarcinomas and other non-squamous malignancies typically show minimal or absent staining, supporting the use of Cystatin A Antibody for IHC in distinguishing squamous tumors from other epithelial cancer types. The ability to visualize these lineage-specific patterns across TMA cores enhances its utility in tumor classification and differential diagnosis studies.
Tissue Microarray (TMA) analysis enables direct side-by-side comparison of CSTA expression across hundreds of tissue cores, demonstrating reproducible staining with strong signal in squamous epithelia and corresponding tumor types, alongside minimal background in non-expressing tissues. The performance of clone MSVA-461M in TMA-based IHC highlights its ability to generate consistent, well-defined cytoplasmic staining across diverse tissue types, supporting its use in large-scale immunohistochemistry studies, biomarker validation, and tissue profiling applications.
This antibody targets Cystatin A in research applications requiring precise and interpretable immunohistochemical detection of epithelial differentiation markers in FFPE tissue sections, making it well suited for studies of squamous cell biology, tumor classification, and epithelial lineage identification.
This antibody is part of the < a href=../cystatin-a-antibody.html>Cystatin antibody collection, where additional CSTA antibodies can be explored.
This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues. -
UniProt
- P01040
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途径
- Response to Water Deprivation
抗原
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