Recombinant F13A1 抗体 (AA 46-181)
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Quick Overview for Recombinant F13A1 抗体 (AA 46-181) (ABIN8093094)
抗原
See all F13A1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 46-181
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原理
- Factor XIIIa Antibody / Coagulation factor XIII A chain
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免疫原
- A recombinant fragment of human Factor XIIIa protein (amino acids 46-181) (exact sequence is proprietary) was used as the immunogen for the F13A1 / Coagulation factor XIII A chain antibody for IHC.
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亚型
- IgG, kappa
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anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) (AA 107-345) antibody
F13A1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) (AA 372-616) antibodyF13A1 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) antibodyF13A1 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3F1 unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) antibodyF13A1 适用: 人 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 9E2 unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) (AA 140-377) antibodyF13A1 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) (N-Term) antibodyF13A1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) (AA 46-181) antibodyF13A1 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 小鼠 Monoclonal F13A1-1448 unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) (AA 170-204) antibodyF13A1 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB57789 unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) (AA 60-94) antibodyF13A1 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB57788 unconjugated
anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) (AA 380-520) antibodyF13A1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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1. Optimal dilution of the F13A1 Antibody for IHC / Coagulation factor XIII A chain should be determined by the researcher.
2. This F13A1 / Coagulation factor XIII A chain antibody is recombinantly produced by expression in human HEK293 cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- F13A1 / Coagulation factor XIII A chain antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.
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- F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))
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别名
- Factor XIIIa
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背景
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F13A1 Antibody for IHC recognizes Coagulation factor XIII A, the catalytic transglutaminase subunit encoded by the F13A1 gene on chromosome 6p25.1. Coagulation factor XIII A, also referred to as Factor XIIIa or Factor XIII A chain, is a cytoplasmic enzyme expressed in platelets, macrophages, dermal dendritic cells, and selected stromal populations. Upon activation by thrombin and calcium, the inactive zymogen is cleaved to generate active Factor XIIIa, which catalyzes covalent cross-linking of fibrin monomers. This reaction stabilizes the fibrin clot and confers resistance to fibrinolysis, representing the final enzymatic step of the coagulation cascade.
Coagulation factor XIII A belongs to the transglutaminase family and contains a catalytic core domain that mediates formation of epsilon-gamma glutamyl-lysine isopeptide bonds between substrate proteins. In plasma, factor XIII circulates as a heterotetramer composed of two catalytic A subunits and two non-catalytic B subunits. Following thrombin-mediated activation, the B subunits dissociate and the A subunits become enzymatically active. Beyond clot stabilization, Factor XIIIa contributes to extracellular matrix remodeling by cross-linking fibronectin, collagen, and other structural proteins, supporting wound healing and tissue repair processes.
In normal tissues, Coagulation factor XIII A expression is prominent in dermal dendrocytes and tissue macrophages. It is also detectable in placenta and bone marrow-derived cells. In fibrohistiocytic lesions, F13A1 Antibody for IHC is frequently applied in research settings to help characterize dermatofibroma, which typically demonstrates strong cytoplasmic positivity in spindle-shaped dermal cells, whereas dermatofibrosarcoma protuberans generally lacks staining. Expression patterns have also been described in inflammatory and reparative conditions involving macrophage activation and stromal remodeling.
Inherited deficiency of Factor XIII results in impaired clot stability and delayed wound healing, underscoring the biologic importance of F13A1 function. Altered expression has been observed in inflammatory and fibrotic disorders, reflecting its broader role in immune cell biology and extracellular matrix stabilization. Clone MSVA-813R is a rabbit monoclonal antibody suitable for detecting Coagulation factor XIII A expression in immunohistochemistry and related research applications, where staining is typically cytoplasmic in dendritic or macrophage-lineage cells.
This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues. -
UniProt
- P00488
抗原
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