TUBB 抗体
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北京 101111
Quick Overview for TUBB 抗体 (ABIN8071385)
抗原
See all TUBB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-beta-Tubulin, mAb (AXO45)
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特异性
- Detects almost all isotypes of beta-tubulin specifically in cells and tissues of mouse, human and ciliates.
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产品特性
- Monoclonal Antibody. Detects almost all isotypes of β-tubulin specifically in cells and tissues of mouse, human and ciliates. Applications: ICC, IHC, IP and WB. Clone: AXO45. Isotype: Mouse IgG1. The β-tubulin monoclonal antibody (AXO45) was developed by isolating the cilia from unicellular multiciliated Paramecium and purifying the core microtubule-based structures of the cilia, the axonemes. These axonemes were electrophoresed on a SDS-PAGE and the tubulin band was used as an antigen to develop monoclonal antibodies targeting both α- and β-tubulin (A.M. Callen, et al., 1994). The antibody AXO45 recognizes the C-terminal region between residues 257 and 442 of β-tubulin. Although the antibody was raised against the β-tubulin found in cilia, it is able to recognize β-tubulin of all microtubule networks, indicating that it can potentially recognize different isotypes of β-tubulin found in mammals.
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纯度
- >95 % (SDS-PAGE)
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免疫原
- Axonemal tubulin from the ciliate Paramecium electroeluted from the 55 kDa band of the SDS-PAGE gel.
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亚型
- IgG1
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anti-Tubulin, beta (TUBB) (AA 40-260) antibody
TUBB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta (TUBB) antibodyTUBB 适用: 人, 小鼠, 大鼠, 小鸡, Pig, Tetrahymena, 鱼, Paramecium, Arabidopsis, Nicotiana tabacum WB, ICC, IHC (p) 宿主: 小鼠 Monoclonal TU-06 unconjugated
anti-Tubulin, beta (TUBB) antibodyTUBB 适用: 人 WB, IF, ICC, IHC (p), IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta (TUBB) (AA 61-160) antibodyTUBB 适用: 人 WB, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta (TUBB) (AA 1-50) antibodyTUBB 适用: 人, 小鼠 WB, IHC, FACS, ICC, IP 宿主: 兔 Monoclonal BLR236K unconjugated Recombinant Antibody
anti-Tubulin, beta (TUBB) (AA 170-419) antibodyTUBB 适用: 人 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Tubulin, beta (TUBB) (AA 170-419) antibodyTUBB 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta (TUBB) (AA 170-419) antibodyTUBB 适用: 人 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal C6 unconjugated
anti-Tubulin, beta (TUBB) antibodyTUBB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 87CT59-3-7 unconjugated
anti-Tubulin, beta (TUBB) (AA 160-447) antibodyTUBB 适用: 人, 小鼠 WB, IHC, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- In PBS containing 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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+4°C
-20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
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有效期
- 12 months
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- TUBB (Tubulin, beta (TUBB))
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别名
- beta-Tubulin
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背景
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Alternate Names/Synonyms: Tubulin beta Chain, Tubulin beta-5 Chain
Product Description: The beta-tubulin monoclonal antibody (AXO45) was developed by isolating the cilia from unicellular multiciliated Paramecium and purifying the core microtubule-based structures of the cilia, the axonemes. These axonemes were electrophoresed on a SDS-PAGE and the tubulin band was used as an antigen to develop monoclonal antibodies targeting both alpha- and beta-tubulin (A.M. Callen, et al., 1994). The antibody AXO45 recognizes the C-terminal region between residues 257 and 442 of beta-tubulin. Although the antibody was raised against the beta-tubulin found in cilia, it is able to recognize beta-tubulin of all microtubule networks, indicating that it can potentially recognize different isotypes of beta-tubulin found in mammals.
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途径
- Microtubule Dynamics, M Phase
抗原
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