IgE 抗体
The Human 单克隆 anti-IgE antibody is suitable to detect IgE in samples from 人. It has been validated for WB, IHC, ELISA 和 St.
产品编号 ABIN8065503
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for IgE 抗体 (ABIN8065503)
抗原
See all IgE 抗体
IgE
适用
人
宿主
Human
克隆类型
单克隆
标记
This IgE antibody is un-conjugated
应用范围
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Stimulation (St)
克隆位点
HE1
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原理
- Anti-Human IgE (non-immune) Antibody
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特异性
- This product is purified human IgE with kappa light chains produced in vitro from a monoclonal hybridoma. Original material is obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B.The purity of the human IgE is > 90 % , tested by SDS-PAGE.As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes.
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预测反应
- Human
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产品特性
- Endotoxin level < 25 EU/mg
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纯化方法
- The human IgE is purified by Protein L chromatography. The remaining contaminants are mainly components from Foetal Bovine Serum
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内毒素水平
- Endotoxin level < 25 EU/mg
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免疫原
- Non-immune
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亚型
- IgE, kappa
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anti-IgE (AA 246-275) antibody
适用: 人 WB 宿主: 兔 Polyclonal RB32377 unconjugated
anti-IgE (AA 1-421) antibody适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 0.01 M phosphate buffer, pH 7.4, containing 0.15 M NaCl.
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储存条件
- 4 °C,-20 °C
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储存方法
- For long term storage, -18 to -22°C is recommended, but it may be stored for short term use at 2 to 8°C without exposure to light. The expiry date refers to storage at -18 to -22°C.
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有效期
- 24 months
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- IgE
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物质类
- Antibody
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背景
- IGHE, IgE, constant region of heavy chain of IgE, immunoglobulin epsilon,Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). {ECO:0000303|PubMed:17576170, ECO:0000303|PubMed:20176268, ECO:0000303|PubMed:22158414}., [Isoform 1]: Constant region of secreted IgE, also known as the Fc region of IgE antibody. Mediates IgE effector functions on myeloid and lymphoid cells primarily via two Fc receptors, the high-affinity IgE Fc receptor complex/FCER1A:MS4A2:FCGR1A and the low-affinity FCER2 receptor, which upon antigen/allergen cross-linking initiate signaling pathways that lead to immune cell activation and differentiation (PubMed:2167225, PubMed:25629393, PubMed:33840121, PubMed:7544003, PubMed:8114916, PubMed:8551243). Triggers the immediate hypersensitivity response to allergens as a host defense mechanism against helminth parasites, pathogenic bacteria and venom toxicity. When dysregulated, it can elicit harmful life-threatening allergic and anaphylactic reactions (PubMed:25629393, PubMed:33840121, PubMed:7544003, PubMed:8114916, PubMed:8551243). Stimulates the high-affinity IgE Fc receptor complex/FCER1A:MS4A2:FCGR1A on mast cells, basophils and eosinophils leading to secretion of vasoactive amines, lipid mediators and cytokines that contribute to inflammatory response, tissue remodeling and cytotoxicity against microbes (PubMed:25629393, PubMed:8114916, PubMed:8551243). On macrophages, cross-linking of FCER2 by IgE immune complexes induces intracellular killing of parasites through activation of L-Arginine-nitric oxide pathway (PubMed:7544003). Activates macrophages to kill tumor cells via antigen-specific antibody-dependent cytotoxicity (ADCC). Triggers differentiation of quiescent M0 macrophages toward M1 state and reprograms M2 macrophages toward a proinflammatory state with antitumor functions (PubMed:30956175). Stimulates FCER2 on B cells and initiates IgE-dependent antigen uptake and presentation to T cells (PubMed:2167225). {ECO:0000269|PubMed:2167225, ECO:0000269|PubMed:25629393, ECO:0000269|PubMed:30956175, ECO:0000269|PubMed:33840121, ECO:0000269|PubMed:7544003, ECO:0000269|PubMed:8114916, ECO:0000269|PubMed:8551243, ECO:0000303|PubMed:20176268, ECO:0000305|PubMed:10917520, ECO:0000305|PubMed:7995941}., [Isoform 2]: Constant region of membrane-bound IgE (long mIgE), part of the B cell receptor complex (BCR). Upon antigen cross-linking triggers quick BCR signaling, ensuring survival of IgE-switched B cells and differentiation into plasma cells, thus regulating both primary and memory IgE responses. {ECO:0000269|PubMed:20458139, ECO:0000269|PubMed:8976175}., [Isoform 3]: Constant region of membrane-bound IgE (short mIgE), part of the B cell receptor complex (BCR). Upon antigen cross-linking initiates slower but sustained BCR signaling that negatively regulates mature B cell proliferation. {ECO:0000269|PubMed:8976175}.,IGHE
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分子量
- 71 kDa (heavy chain), 24 kDa (light chain)
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基因ID
- 3497
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UniProt
- P01854
抗原
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