LRRC75A/FAM211A 抗体 (AA 52-292) (Fluoro647)
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Quick Overview for LRRC75A/FAM211A 抗体 (AA 52-292) (Fluoro647) (ABIN8005565)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 52-292
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原理
- Anti-LRRC75A Antibody Fluoro647 Conjugated
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交叉反应 （详细）
- No cross reactivity with other proteins.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human LRRC75A recombinant protein (Position: H52-Q292).
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亚型
- IgG
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应用备注
- Flow Cytometry, Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
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有效期
- 12 months
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- LRRC75A/FAM211A (LRRC75A) (Leucine Rich Repeat Containing 75A (LRRC75A))
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别名
- LRRC75A
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背景
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Background: Long noncoding RNAs (lncRNAs) play multiple key roles during inflammatory processes. A novel lncRNA identified by the high-throughput sequencing analysis was found significantly down-regulated in Escherichia coli-introduced cell model of bovine mastitis. Given that this lncRNA consists of the antisense of leucine-rich repeat-containing protein 75A (LRRC75A), it was named LRRC75A antisense lncRNA1 (LRRC75A-AS1). The expression of LRRC75A-AS1 was down-regulated in bovine mammary epithelial cells and mammary tissues under inflammatory condition. Knockout (KO) of LRRC75A-AS1 by CRISPR-Cas9 system in bovine mammary alveolar cell-T (MAC-T) cell line could enhance expressions of tight junction (TJ) proteins Claudin-1, Occludin and ZO-1, reduce cell monolayer permeability, and inhibit Staphylococcus aureus adhesion and invasion. Meanwhile, it also down-regulated expressions of inflammatory factors and attenuated activation of NF-κB pathway. Similarly, knockdown of LRRC75A caused the changes as LRRC75A-AS1 KO did, while overexpression of LRRC75A enabled the opposite effects. TJ of epithelioid cells barriers the pathogenic microorganisms outside during inflammation, in which LRRC75A-AS1 can regulate the expression of TJ proteins through LRRC75A, affecting the development of inflammation.
Gene Full Name: leucine rich repeat containing 75A
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基因ID
- 388341
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UniProt
- Q8NAA5
抗原
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