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BUB3 抗体 (Middle Region) (Biotin)

The 兔 多克隆 anti-BUB3 antibody is suitable to detect BUB3 in samples from 人, 小鼠 和 大鼠. It has been validated for WB, ELISA 和 IHC.
产品编号 ABIN7973488
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for BUB3 抗体 (Middle Region) (Biotin) (ABIN7973488)

抗原

See all BUB3 抗体
BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

适用

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人, 小鼠, 大鼠

宿主

  • 98
  • 2
  • 1

克隆类型

  • 80
  • 21
多克隆

标记

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This BUB3 antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • 抗原表位

    • 15
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    Middle Region

    原理

    Anti-Bub3 Antibody Biotin Conjugated

    特异性

    No cross reactivity with other proteins.

    交叉反应 (详细)

    No cross-reactivity with other proteins

    预测反应

    identical to the related rat,mouse sequences.

    纯化方法

    Immunogen affinity purified.

    免疫原

    A synthetic peptide corresponding to a sequence in the middle region of human Bub3, identical to the related rat and mouse sequences.

    亚型

    IgG
  • 应用备注

    Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

    有效期

    12 months
  • 抗原

    BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

    别名

    BUB3

    背景

    Background: BUB3 (BUDDING UNINHIBITED BY BENZIMIDAZOLES 3) is a protein that in humans is encoded by the BUB3 gene. And it is mapped on 10q26.13. Bub3 is also a protein involved with the regulation of the Spindle Assembly Checkpoint (SAC), though BUB3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, Bub3 delays the irreversible onset of anaphase through ion of kinetochore localization during prometaphase to achieve biorentation. Bub3 s the localization of Bub1 at the kinetochore in order to activate the SAC. Bub3 also acts as a regulator in that it affects binding of Mad3 to Mad2. Using deletion analysis, the authors identified a domain of BUB1 that is required both for binding BUB3 and for kinetochore localization of BUB1.

    Gene Full Name: BUB3 mitotic checkpoint protein

    Sequence Similarities: Belongs to the WD repeat BUB3 family.

    基因ID

    9184

    UniProt

    O43684

    途径

    Maintenance of Protein Location
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