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SUZ12 抗体 (C-Term)

The 兔 多克隆 anti-SUZ12 antibody (ABIN7938516) specifically detects SUZ12 in WB, IF, ChIP, ChIP-seq 和 CUT&RUN. The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN7938516
发货至: 中国
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Quick Overview for SUZ12 抗体 (C-Term) (ABIN7938516)

抗原

See all SUZ12 抗体
SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

适用

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人, 小鼠

宿主

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克隆类型

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多克隆

标记

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This SUZ12 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
  • 抗原表位

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    C-Term

    原理

    Suz12 antibody (pAb)

    纯化方法

    Affinity Purified

    免疫原

    This Suz12 antibody was raised against a peptide corresponding to the C-terminus of human Suz12.

    亚型

    IgG
  • 应用备注

    ChIP: 10 µL per ChIP ChIP-Seq: 10 µL each WB*: 1:250 - 1:1,000 dilution CUT&RUN: 1 µL per 50 µL reaction *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. An additional band at approximately 20 kDa is also detected by this antibody in HeLa and HEK lysates, but not in mouse CSML0 cell lysate. For Suz12, we also offer AbFlex Suz12 Recombinant Antibody (rAb). For details, see Catalog No. 91163.

    限制

    仅限研究用
  • 缓冲液

    PBS, 30 % glycerol, 0.035 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

    储存条件

    -20 °C

    储存方法

    Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

    有效期

    24 months
  • 抗原

    SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

    别名

    Suz12

    背景

    Background_Plain: Suz12 (Suppressor-of-Zeste 12 protein, also designated as ChET9, JJAZ1 protein) is a component of the Polycomb group complexes 2, 3 and 4 (PRC2, PRC3 and PRC4), which also contain the histone methyltransferase EZH2, EED (Embryonic Ectoderm Development protein) protein and the histone-binding proteins RbAP46 and RbAP48. PRC2 is responsible for di- and trimethylation of histone 3 at lysine 27 (Histone H3K27me2 and Histone H3K27me3), which leads to transcriptional repression of the target genes. Suz12 is a nuclear protein belonging to the VEFS family. Suz12 contains a zinc-finger binding motif and a VEFS box, which is required for the interaction between Suz12 and EZH2.

    Aliases: CHET9, JJAZ1

    分子量

    95 kDa

    NCBI登录号

    NP_056170
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