SUPT5H 抗体 (C-Term)
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Quick Overview for SUPT5H 抗体 (C-Term) (ABIN7938513)
抗原
See all SUPT5H 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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原理
- Supt5H/SPT5 antibody (mAb) (Clone 6F1)
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纯化方法
- Protein G Chromatography
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免疫原
- This antibody was raised against a synthetic peptide derived from the human SPT5 C-terminal region.
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亚型
- IgG1
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应用备注
- ChIP-Seq: 4 μg per ChIP WB*: 0.5 - 2 μg/mL IP: 5 μg/mL *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91331.
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限制
- 仅限研究用
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))
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别名
- Supt5H/SPT5
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背景
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Background_Plain: SUPT5H (SPT5 Homolog, DSIF Elongation Factor Subunit) is a transcription elongation factor which regulates mRNA processing and transcription elongation by RNA polymerase II. SUPT5H positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. SUPT5H also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Diseases associated with SUPT5H include HIV-1. Among its related pathways are mRNA capping and HIV life cycle.
Aliases: SPT5 Homolog, DSIF Elongation Factor Subunit, DSIF Large Subunit, Transcription Elongation Factor SPT5
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分子量
- 140 kDa
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NCBI登录号
- NP_001104490
抗原
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