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SUPT5H 抗体 (C-Term)

The 大鼠 单克隆 anti-SUPT5H antibody is suitable to detect SUPT5H in samples from 人. It has been validated for WB, IP 和 ChIP-seq.
产品编号 ABIN7938512
发货至: 中国
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Quick Overview for SUPT5H 抗体 (C-Term) (ABIN7938512)

抗原

See all SUPT5H 抗体
SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

适用

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宿主

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大鼠

克隆类型

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单克隆

标记

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This SUPT5H antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq)

克隆位点

6F1
  • 抗原表位

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    C-Term

    原理

    Supt5H/SPT5 antibody (mAb) (Clone 6F1)

    纯化方法

    Protein G Chromatography

    免疫原

    This antibody was raised against a synthetic peptide derived from the human SPT5 C-terminal region.

    亚型

    IgG1
  • 应用备注

    ChIP-Seq: 4 μg per ChIP WB*: 0.5 - 2 μg/mL IP: 5 μg/mL *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91331.

    限制

    仅限研究用
  • 缓冲液

    Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

    储存条件

    -20 °C

    储存方法

    Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

    有效期

    24 months
  • 抗原

    SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

    别名

    Supt5H/SPT5

    背景

    Background_Plain: SUPT5H (SPT5 Homolog, DSIF Elongation Factor Subunit) is a transcription elongation factor which regulates mRNA processing and transcription elongation by RNA polymerase II. SUPT5H positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. SUPT5H also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Diseases associated with SUPT5H include HIV-1. Among its related pathways are mRNA capping and HIV life cycle.

    Aliases: SPT5 Homolog, DSIF Elongation Factor Subunit, DSIF Large Subunit, Transcription Elongation Factor SPT5

    分子量

    140 kDa

    NCBI登录号

    NP_001104490
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