POLR2F 抗体 (meLys7)
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北京 101111
Quick Overview for POLR2F 抗体 (meLys7) (ABIN7938446)
抗原
See all POLR2F 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- meLys7
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原理
- RNA pol II CTD monomethyl Lys7 antibody (mAb) (Clone 1F5)
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纯化方法
- Protein G Chromatography
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免疫原
- This antibody was raised against a peptide containing dimethyl-lysine 7 of human RNA Pol II CTD.
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亚型
- IgG2a
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anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 1-127) antibody
POLR2F 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) antibodyPOLR2F 适用: 人 WB, IF, ChIP, ChIP-seq, ICC 宿主: 大鼠 Monoclonal 1F4B6 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (N-Term) antibodyPOLR2F 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) antibodyPOLR2F 适用: 人, 大鼠, 小鼠, C. elegans, Saccharomyces cerevisiae WB, IF, ChIP, ChIP-seq, ICC 宿主: 小鼠 Monoclonal 4H8 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 27-61) antibodyPOLR2F 适用: 小鼠 WB 宿主: 兔 Polyclonal RB56843 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 1-127) antibodyPOLR2F 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) antibodyPOLR2F 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- ChIP-Seq: 10 μg per ChIP IP: 5 μg per IP WB*: 0.5 - 2 μg/mL *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
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限制
- 仅限研究用
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缓冲液
- PBS containing 0.05 % BSA and 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))
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别名
- RNA pol II
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背景
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Background_Plain: Genome-wide profiling revealed high levels of K7me1/2 marks at the transcriptional start site of genes for sense and antisense transcribing RNAPII. The new K7 modifications further expand the mammalian CTD code to allow regulation of differential gene expression. In analogy, it appears likely that the various modifications of K7 residues identified in this analysis may similarly contribute to fine tuning of gene expressing by RNAPII, and that these modifications are placed in K7 residues of CTD dependent on cell signaling and epigenetic programming of cells.
Aliases: RNA Polymerase II Carboxy Terminal Domain
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分子量
- 240 kDa
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NCBI登录号
- NP_000928
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途径
- Regulatory RNA Pathways
抗原
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