POLR2F 抗体
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北京 101111
Quick Overview for POLR2F 抗体 (ABIN7938437)
抗原
See all POLR2F 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- RNA pol II antibody (mAb) (Clone 4H8)
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纯化方法
- Affinity Purified
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免疫原
- This RNA pol II antibody was raised against a synthetic peptide YSPTSpPS corresponding to human RNA pol II. It recognizes both the phosphorylated and unphosphorylated forms of the largest subunit of RNA pol II.
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亚型
- IgG1
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anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 1-127) antibody
POLR2F 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) antibodyPOLR2F 适用: 人 WB, IF, ChIP, ChIP-seq, ICC 宿主: 大鼠 Monoclonal 1F4B6 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (N-Term) antibodyPOLR2F 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) antibodyPOLR2F 适用: 人, 大鼠, 小鼠, C. elegans, Saccharomyces cerevisiae WB, IF, ChIP, ChIP-seq, ICC 宿主: 小鼠 Monoclonal 4H8 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 27-61) antibodyPOLR2F 适用: 小鼠 WB 宿主: 兔 Polyclonal RB56843 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 1-127) antibodyPOLR2F 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) antibodyPOLR2F 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- ChIP: 10 µL per ChIP ChIP-Seq: 6-20 µL each WB: 1:2,000 - 1:5,000 dilution For optimal results in Western blotting, primary antibody incubations should be performed overnight at 4 °C. The addition of 0.1 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
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限制
- 仅限研究用
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缓冲液
- PBS containing 0.2 % gelatin, 0.01 % thimerosal, 0.1 % sodium azide, and 50 % glycerol. Thimerosal and sodium azide are highly toxic.
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储存液
- Sodium azide, Thimerosal (Merthiolate)
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注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))
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别名
- RNA pol II
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背景
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Background_Plain: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Aliases: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
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分子量
- 217 kDa
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NCBI登录号
- NP_000928
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途径
- Regulatory RNA Pathways
抗原
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