PCIF1 抗体 (C-Term)
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Quick Overview for PCIF1 抗体 (C-Term) (ABIN7938398)
抗原
See all PCIF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- PCIF1/CAPAM antibody (pAb)
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纯化方法
- Protein A Chromatography
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免疫原
- This antibody was raised against a peptide within the C-terminal region of human PCIF1.
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亚型
- IgG
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anti-PDX1 C-terminal Inhibiting Factor 1 (PCIF1) (AA 1-704) antibody
PCIF1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-PDX1 C-terminal Inhibiting Factor 1 (PCIF1) (AA 201-500) antibodyPCIF1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-PDX1 C-terminal Inhibiting Factor 1 (PCIF1) (AA 654-704) antibodyPCIF1 适用: 人, 小鼠 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-PDX1 C-terminal Inhibiting Factor 1 (PCIF1) (Middle Region) antibodyPCIF1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- IP: 10 µL per IP WB*: 0.5-2 μg/mL *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For optimal IP results, the addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
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限制
- 仅限研究用
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缓冲液
- Purified IgG in PBS, 30 % glycerol, and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- PCIF1 (PDX1 C-terminal Inhibiting Factor 1 (PCIF1))
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别名
- PCIF1
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背景
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Background_Plain: The abundant modification N6-methyladenosine (m6A), in eukarytic mRNAs, has been identified as a major epitranscriptome mark that plays critical roles in RNA metabolism and function. In addition to the internal m6A, N6, 2-O dimethyladenosine (m6Am) is present at the transcription start site of capped mRNAs in vertebrates. The abundance of this modification in the transcriptome has been quantified and identified as the writer protein, cap-specific adenosine methyltransferase (CAPAM/PCIF1), is needed for this modification. CAPAM/PCIF1 is identified as the Methyltransferase responsible for methylating 2-O-methyladenosine (Am) at its N6 position to generate m6Am. m6Am is involved in regulating translation of capped mRNAs. Depleting CAPAM/PCIF1 does not affect mRNA translation but is associated with reduced stability of a subset of m6Am annotated mRNAs.
Aliases: Transcription factor PCF1, Phosphorylated CTD Interacting Factor 1 (PCIF1)
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分子量
- 80 kDa
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NCBI登录号
- NP_071387
抗原
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