BRD9 抗体 (C-Term)
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Quick Overview for BRD9 抗体 (C-Term) (ABIN7938073)
抗原
See all BRD9 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- BRD9 antibody (pAb), sample
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纯化方法
- Affinity Purified
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免疫原
- This antibody was raised against a peptide within the C-terminal region of human BRD9 (bromodomain containing 9).
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亚型
- IgG
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应用备注
- IP: 5 µL per IP WB*: 1:250 - 1:1,000 dilution *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For optimal IP results, the addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
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限制
- 仅限研究用
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- BRD9 (Bromodomain Containing 9 (BRD9))
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别名
- BRD9
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背景
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Background_Plain: BRD9 (bromodomain containing 9) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6) that function in the regulation of transcriptional activation and chromatin remodeling. There are five isoforms of BRD9 that are produced by alternative splicing.
Aliases: PRO9856, LAVS3040
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分子量
- 85 kDa
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NCBI登录号
- NP_076413
抗原
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