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BRD9 抗体 (C-Term)

The 兔 多克隆 anti-BRD9 antibody is suitable to detect BRD9 in samples from 人. It has been validated for WB 和 IP.
产品编号 ABIN7938071
发货至: 中国
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Quick Overview for BRD9 抗体 (C-Term) (ABIN7938071)

抗原

See all BRD9 抗体
BRD9 (Bromodomain Containing 9 (BRD9))

适用

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宿主

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克隆类型

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多克隆

标记

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This BRD9 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP)
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    C-Term

    原理

    BRD9 antibody (pAb)

    纯化方法

    Affinity Purified

    免疫原

    This antibody was raised against a peptide within the C-terminal region of human BRD9 (bromodomain containing 9).

    亚型

    IgG
  • 应用备注

    IP: 5 µL per IP WB*: 1:250 - 1:1,000 dilution *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For optimal IP results, the addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

    限制

    仅限研究用
  • 缓冲液

    Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

    储存条件

    -20 °C

    储存方法

    Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

    有效期

    24 months
  • 抗原

    BRD9 (Bromodomain Containing 9 (BRD9))

    别名

    BRD9

    背景

    Background_Plain: BRD9 (bromodomain containing 9) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6) that function in the regulation of transcriptional activation and chromatin remodeling. There are five isoforms of BRD9 that are produced by alternative splicing.

    Aliases: PRO9856, LAVS3040

    分子量

    85 kDa

    NCBI登录号

    NP_076413
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