CLCN3 抗体 (C-Term, Intracellular)
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Quick Overview for CLCN3 抗体 (C-Term, Intracellular) (ABIN7884874)
抗原
See all CLCN3 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 592-661, C-Term, Intracellular
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原理
- A Rabbit Polyclonal Antibody to CLC-3 (CLCN3) Channel
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预测反应
- rat CLC-5 - 49,70 amino acid residues identicalRat CLC-4 - 46, human,70 amino acid residues identical,rabbit,guinea pig - 69,Mouse - identical, Xenopus laevis - 61
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纯化方法
- The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.
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免疫原
- GST fusion protein with the sequence SLVVIVFELTGGLEYIVPLMAAVMTSKWVGDAFGREGIYEAHIRLNGYPFLDAKEEFTHTTLAADVMRPR, corresponding to amino acid residues 592-661 of rat CLC-3
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亚型
- IgG
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anti-Chloride Channel 3 (CLCN3) (C-Term) antibody
CLCN3 适用: 大鼠, 人, 小鼠, 豚鼠, 兔, 犬, 马, Cow, 斑马鱼, 绵羊 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chloride Channel 3 (CLCN3) (AA 98-115) antibody (Atto 390)CLCN3 适用: 大鼠 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S258-5 Atto 390
anti-Chloride Channel 3 (CLCN3) (AA 98-115) antibody (Atto 594)CLCN3 适用: 大鼠 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S258-5 Atto 594
anti-Chloride Channel 3 (CLCN3) (AA 98-115) antibody (APC)CLCN3 适用: 大鼠 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S258-5 APC
anti-Chloride Channel 3 (CLCN3) (AA 98-115) antibody (Atto 488)CLCN3 适用: 大鼠 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S258-5 Atto 488
anti-Chloride Channel 3 (CLCN3) (AA 98-115) antibody (Biotin)CLCN3 适用: 大鼠 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S258-5 Biotin
anti-Chloride Channel 3 (CLCN3) (AA 98-115) antibody (HRP)CLCN3 适用: 大鼠 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S258-5 HRP
anti-Chloride Channel 3 (CLCN3) (AA 98-115) antibody (PerCP)CLCN3 适用: 大鼠 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S258-5 PerCP
anti-Chloride Channel 3 (CLCN3) (AA 98-115) antibody (FITC)CLCN3 适用: 大鼠 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S258-5 FITC
anti-Chloride Channel 3 (CLCN3) (AA 98-115) antibody (PE)CLCN3 适用: 大鼠 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S258-5 PE
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应用备注
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WB: 1:200
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: The optimal concentration should be determined by the user
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7235086)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW)
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- CLCN3 (Chloride Channel 3 (CLCN3))
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别名
- Chloride channel 3
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背景
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Synonyms: Chloride channel 3, Chloride transporter ClC-3, H+/Cl- exchange transporter 3
Description: CLC-3 is a member of the voltage-dependent Cl- channel (CLC) family that includes nine known members in mammals. CLC channels can be classified as plasma membrane channels and intracellular organelle channels. The first group includes the CLC-1, CLC-2 CLC-Ka and CLCKb channels. The second group comprises the CLC-3, CLC-4, CLC-5, CLC-6 and CLC-7.CLC channels that function in the plasma membrane are involved in the stabilization of membrane potential and in transepithelial transport. The presumed function of the intracellular CLC channels is support of the acidification of the intraorganellar compartment. In this regard, recent reports indicate that ClC-4 and ClC-5 (and by inference ClC-3) can function as Cl-/H+ antiporters.1, 2The functional unit of the CLC channels is a dimer with each subunit forming a proper pore. Although the crystal structure of bacterial CLC channels was resolved, the topology of the CLC channels is complex and has not been fully elucidated. It is generally accepted that both the N- and C- terminus domains are intracellular while the number and configuration of the transmembrane domains vary greatly between different models. 1,2CLC-3 is widely distributed with prominent expression in tissues of neuroectoderm origin. In the brain, it is highly expressed in the hippocampus, olfactory bulb and olfactory cortex. The channel is also prominently expressed in aortic and coronary vascular smooth muscle cells, aortic endothelial cells and tracheal and alveolar epithelial cells.The physiological function of CLC-3 is not entirely clear, but it has been suggested that CLC-3 generates a shunt current of chloride for v-H+-ATPases, thereby aiding the acidification of endosomes and synaptic vesicles as well as lysosomes. Disruption of the ClC-3 gene in mice causes severe neuronal loss, leading to a complete loss of the hippocampus in adult mice. In addition, CLC-3 has been shown to have a critical role in the respiratory burst and phagocytosis of polymorphonuclear cells, a key cell type of innate host defense. 3,4
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基因ID
- 84360
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UniProt
- P51792
抗原
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