GHRH 抗体 (AA 1-29)
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北京 101111
Quick Overview for GHRH 抗体 (AA 1-29) (ABIN7884242)
抗原
See all GHRH 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-29
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原理
- Growth Hormone-Releasing Hormone Sheep Polyclonal Antibody
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特异性
- The antibody recognizes the rat GHRH and it shows no cross-reactivity with SRIF-14, SRIF-28 rGH, LH, FSH, PRL calcitonin, insulin, glucagon ovine CRF, secretin, vasoactive intestinal peptide, and cholecystokinin-8
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免疫原
- synthetic rat GHRH (1-29)NH2
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 50 μL: IHC: 1:15000, In vivo Passive Immunization: neat serum, RIA: In a RIA for rGRF-(1043), at a final dilution of 1: 785,000, this antiserum binds 35 % of [125I]-rGRF and can detect 7pg/assay tube. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized serum
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- GHRH (Growth Hormone Releasing Hormone (GHRH))
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别名
- Growth Hormone-Releasing Hormone
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背景
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GHRH, Somatocrinin, Somatoliberin, GH-RH, Growth hormone-releasing factor (GHRF or GRF), Somatotropin-releasing hormone (SRH), Somatotropin-releasing factor (SRF), Somatorelin
GHRH belongs to the glucagon family produced in the hypothalamus. It is essential for normal linear growth and has important metabolic effects throughout life. GHRH stimulates GH production and release by binding to the GHRH Receptor (GHRHR) on cells in the anterior pituitary. GHRH is released from neurosecretory nerve terminals of arcuate neurons, and is carried by the hypothalamo-hypophyseal portal system to the anterior pituitary gland, where it stimulates growth hormone(GH) secretion by stimulating the growth hormone-releasing hormone receptor.
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途径
- Hormone Transport, Hormone Activity, cAMP Metabolic Process
抗原
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