DBH 抗体
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北京 101111
Quick Overview for DBH 抗体 (ABIN7884240)
抗原
See all DBH 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Dopamine Beta Hydroxylase (DBH) Mouse Monoclonal Antibody (DBH41)
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特异性
- This antibody recognizes This antibody specifically recognizes two bands of molecular weight 70 and 75 kDa, corresponding to the soluble and membrane bound forms of the enzyme, respectively. Due to the high sensitivity of the DBH antibody, the presence of the enzyme can be immunologically detected even in areas where only scattered DBH-containing fibers are present. DBH antibody specifically stains DBH-containing neurons and fibers in the rat central nervous system (CNS). It exhibits no cross-reactivity with either mouse, human, rabbit, guinea pig, cat or bovine DBH
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免疫原
- Rat DBH
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亚型
- IgG1
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 2 ml: IHC and ICC: 1:30 - 1:50. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized tissue culture supernatant
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))
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别名
- Dopamine Beta Hydroxylase
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背景
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Dopamine beta-monooxygenase Dopamine Beta Hydroxylase, Dopamine-hydroxylase, DBH, oxygenase, anti-Dopamine Beta Hydroxylase, anti-Dopamine-hydroxylase, anti-DBH, oxygenase
Studying the monoaminergic system has been of great interest and importance since dopamine, noradrenaline and adrenaline are the neurotransmitters involved in multiple physiological and pathophysiological conditions. DBH is the enzyme responsible for the biosynthetic hydroxylation of dopamine to noradrenaline.
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途径
- Carbohydrate Homeostasis
抗原
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