Recombinant WDR4 抗体
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Quick Overview for Recombinant WDR4 抗体 (ABIN7882696)
抗原
See all WDR4 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- WDR4 Antibody / WD repeat domain 4
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纯化方法
- Affinity-chromatography
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免疫原
- A synthesized peptide derived from human WDR4 was used as the immunogen for the WDR4 antibody.
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亚型
- IgG
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anti-WD Repeat Domain 4 (WDR4) (AA 1-266) antibody
WDR4 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1F9 unconjugated
anti-WD Repeat Domain 4 (WDR4) (AA 1-412) antibodyWDR4 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-WD Repeat Domain 4 (WDR4) antibodyWDR4 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-WD Repeat Domain 4 (WDR4) (C-Term) antibodyWDR4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-WD Repeat Domain 4 (WDR4) (C-Term) antibodyWDR4 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-WD Repeat Domain 4 (WDR4) antibodyWDR4 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-WD Repeat Domain 4 (WDR4) antibodyWDR4 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB4100 unconjugated Recombinant Antibody
anti-WD Repeat Domain 4 (WDR4) (AA 225-275) antibodyWDR4 适用: 人 IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-WD Repeat Domain 4 (WDR4) (AA 36-85) antibodyWDR4 适用: 人, 马, 犬, 豚鼠, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-WD Repeat Domain 4 (WDR4) (N-Term) antibodyWDR4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the WDR4 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the WDR4 antibody at -20oC.
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- WDR4 (WD Repeat Domain 4 (WDR4))
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别名
- WDR4
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背景
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WDR4 antibody detects WD repeat domain 4, a conserved protein involved in RNA modification and tRNA methylation. WDR4 forms a complex with METTL1 to catalyze the N7-methylguanosine modification of tRNAs, a modification required for translational fidelity and RNA stability. The WDR4 antibody is widely used in molecular biology and RNA research to study RNA methylation, protein translation, and gene expression regulation.
WDR4 is encoded by the WDR4 gene located on human chromosome 21q22.3. The protein is approximately 412 amino acids long and contains several WD40 repeats that mediate protein-protein interactions, forming a beta-propeller structure typical of the WD repeat family. WDR4 localizes to the nucleus and cytoplasm, where it partners with METTL1 to catalyze methylation of specific tRNA guanine residues, ensuring proper translation initiation and elongation.
The WDR4 antibody detects a 46 kilodalton protein by western blot and demonstrates nuclear and cytoplasmic staining patterns under immunofluorescence. Through its interaction with METTL1, WDR4 stabilizes the tRNA methyltransferase complex and regulates global protein synthesis. Loss of WDR4 leads to reduced tRNA methylation, impaired translation, and increased sensitivity to stress.
Mutations in WDR4 cause a spectrum of developmental and neurological disorders, including microcephaly and intellectual disability. These defects arise from disrupted tRNA modification, which affects the translation of growth-related and neuronal genes. In cancer, overexpression of WDR4 enhances translation of oncogenic proteins and promotes tumor progression through metabolic reprogramming.
Because of its key role in RNA modification and translation regulation, WDR4 serves as an essential model for understanding epitranscriptomic control of gene expression. NSJ Bioreagents provides a validated WDR4 antibody optimized for its applications, supporting studies of RNA methylation, translational regulation, and disease pathogenesis. -
UniProt
- P57081
抗原
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