Recombinant VAPA 抗体
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北京 101111
Quick Overview for Recombinant VAPA 抗体 (ABIN7882659)
抗原
See all VAPA 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- VAPA Antibody / Vesicle-associated membrane protein-associated protein A
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纯化方法
- Affinity chromatography
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免疫原
- A synthesized peptide derived from human VAPA was used as the immunogen for the VAPA antibody.
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亚型
- IgG
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anti-VAMP (Vesicle-Associated Membrane Protein)-Associated Protein A, 33kDa (VAPA) (AA 1-242) antibody
VAPA 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4C12 unconjugated
anti-VAMP (Vesicle-Associated Membrane Protein)-Associated Protein A, 33kDa (VAPA) (N-Term) antibodyVAPA 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-VAMP (Vesicle-Associated Membrane Protein)-Associated Protein A, 33kDa (VAPA) antibodyKD Validated VAPA 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB2580 unconjugated Recombinant Antibody
anti-VAMP (Vesicle-Associated Membrane Protein)-Associated Protein A, 33kDa (VAPA) antibodyVAPA 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-VAMP (Vesicle-Associated Membrane Protein)-Associated Protein A, 33kDa (VAPA) (AA 79-92) antibodyVAPA 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-VAMP (Vesicle-Associated Membrane Protein)-Associated Protein A, 33kDa (VAPA) (AA 137-227) antibodyVAPA 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-VAMP (Vesicle-Associated Membrane Protein)-Associated Protein A, 33kDa (VAPA) (AA 125-175) antibodyVAPA 适用: 人, 小鼠 WB, IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-VAMP (Vesicle-Associated Membrane Protein)-Associated Protein A, 33kDa (VAPA) (AA 182-210), (C-Term) antibodyVAPA 适用: 人 WB 宿主: 兔 Polyclonal RB40543 unconjugated
anti-VAMP (Vesicle-Associated Membrane Protein)-Associated Protein A, 33kDa (VAPA) (AA 1-249) antibodyVAPA 适用: 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-VAMP (Vesicle-Associated Membrane Protein)-Associated Protein A, 33kDa (VAPA) (AA 101-200) antibodyVAPA 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the VAPA antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the VAPA antibody at -20oC.
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- VAPA (VAMP (Vesicle-Associated Membrane Protein)-Associated Protein A, 33kDa (VAPA))
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别名
- VAPA
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背景
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VAPA antibody detects Vesicle-associated membrane protein-associated protein A, encoded by the VAPA gene. Vesicle-associated membrane protein-associated protein A is an integral membrane protein localized mainly to the endoplasmic reticulum and involved in membrane trafficking, lipid transfer, and inter-organelle communication. It is part of the VAP protein family, which also includes VAPB, and plays a role in tethering endoplasmic reticulum membranes to other organelles, including the Golgi, mitochondria, and plasma membrane. VAPA antibody provides researchers with a critical tool to study intracellular transport, organelle homeostasis, and lipid signaling.
Vesicle-associated membrane protein-associated protein A interacts with multiple partners through its major sperm protein domain, including proteins containing FFAT motifs that mediate lipid exchange at membrane contact sites. Research using VAPA antibody has demonstrated that these contacts allow the direct transfer of lipids such as phosphoinositides, ceramides, and cholesterol between membranes, bypassing vesicular trafficking. This function is essential for maintaining lipid composition, signaling, and organelle identity. Dysregulation of these processes contributes to neurodegeneration, metabolic syndromes, and viral infection.
In virology, VAPA has been implicated in the life cycles of several viruses, including hepatitis C virus and enteroviruses, which hijack VAPA-dependent contact sites to establish replication organelles. Studies with VAPA antibody have shown that viral proteins interact with Vesicle-associated membrane protein-associated protein A to remodel endoplasmic reticulum membranes, creating environments conducive to viral replication. These findings highlight the relevance of VAPA in host-pathogen interactions and antiviral drug development.
Beyond infectious disease, VAPA is also significant in neurodegeneration and metabolic regulation. Mutations or dysfunction in VAP proteins are linked to motor neuron diseases such as amyotrophic lateral sclerosis, and research using VAPA antibody has explored whether VAPA contributes to similar pathogenic mechanisms. In lipid metabolism, altered expression of VAPA affects cholesterol and sphingolipid distribution, linking it to metabolic disease and cardiovascular risk. These wide-ranging roles underscore the importance of studying VAPA in both normal physiology and pathology.
VAPA antibody is widely used in western blotting, immunofluorescence, and immunoprecipitation. Western blotting reveals its expression in tissues such as brain, liver, and muscle. Immunofluorescence confirms its localization to endoplasmic reticulum membranes, often colocalizing with markers such as calnexin. Immunoprecipitation with VAPA antibody enables identification of interacting proteins, revealing new partners in lipid transfer and signaling pathways. These applications make it a versatile reagent across molecular and cellular studies.
By supplying validated VAPA antibody reagents, NSJ Bioreagents supports research into membrane contact sites, lipid metabolism, and host-pathogen interactions. Detection of Vesicle-associated membrane protein-associated protein A provides a foundation for investigating fundamental processes in cell biology and disease mechanisms. -
UniProt
- Q9P0L0
抗原
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