CD43 抗体
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北京 101111
Quick Overview for CD43 抗体 (ABIN7882270)
抗原
See all CD43 (SPN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- CD43 Antibody
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纯化方法
- Protein G affinity chromatography
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免疫原
- Myeloblastic KG1 cells were used as the immunogen for this CD43 antibody.
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亚型
- IgG1, kappa
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anti-Sialophorin (SPN) antibody
SPN 适用: 人 FACS, WB, IHC, IF 宿主: 小鼠 Monoclonal DF-T1 unconjugated
anti-Sialophorin (SPN) (AA 276-400) antibodySPN 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Sialophorin (SPN) antibodySPN 适用: 人 FACS, WB, IHC (p), IP, Func 宿主: 小鼠 Monoclonal MEM-59 unconjugated
anti-Sialophorin (SPN) antibodySPN 适用: 人 FACS, WB, IHC (p), IP 宿主: 小鼠 Monoclonal MEM-59 unconjugated
anti-Sialophorin (SPN) antibodySPN 适用: 人 FACS, WB, IHC, IF 宿主: 小鼠 Monoclonal rSPN-839 unconjugated Recombinant Antibody
anti-Sialophorin (SPN) antibodySPN 适用: 人 FACS, WB, IHC, IF 宿主: 兔 Monoclonal SPN-1766R unconjugated Recombinant Antibody
anti-Sialophorin (SPN) (AA 272-400) antibodySPN 适用: 人, 小鼠, 大鼠 FACS, WB, IHC 宿主: 小鼠 Monoclonal 4I3 unconjugated
anti-Sialophorin (SPN) antibodySPN 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sialophorin (SPN) (AA 1-400) antibodySPN 适用: 人 FACS, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Sialophorin (SPN) (AA 20-253) antibodySPN 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 1H8B10 unconjugated
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应用备注
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The optimal dilution of the CD43 antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the CD43 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- CD43 (SPN) (Sialophorin (SPN))
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别名
- CD43
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背景
- It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43. 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
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UniProt
- P16150
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途径
- Regulation of Leukocyte Mediated Immunity
抗原
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