Secretory Component 抗体
The 小鼠 单克隆 anti-Secretory Component antibody is suitable to detect Secretory Component in samples from 人. It has been validated for IHC (p).
产品编号 ABIN7882222
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for Secretory Component 抗体 (ABIN7882222)
抗原
Secretory Component
适用
人
宿主
小鼠
克隆类型
单克隆
标记
This Secretory Component antibody is un-conjugated
应用范围
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
克隆位点
SPM217
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原理
- Secretory Component Antibody / ECM1
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纯化方法
- Protein G affinity chromatography
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免疫原
- Secretory Component protein isolated from human colostrum was used as the immunogen for this Secretory Component antibody.
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亚型
- IgG1, kappa
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应用备注
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The optimal dilution of the Secretory Component antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Secretory Component antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Secretory Component
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背景
- This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
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UniProt
- Q16610
抗原
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