Recombinant RASEF 抗体
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北京 101111
Quick Overview for Recombinant RASEF 抗体 (ABIN7882137)
抗原
See all RASEF 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- RASEF Antibody / RAB45 / Ras and EF-hand domain-containing protein
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纯化方法
- Affinity-chromatography
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免疫原
- A synthesized peptide derived from human RAB45 was used as the immunogen for the RASEF antibody.
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亚型
- IgG
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anti-RAS and EF Hand Domain Containing (RASEF) (AA 631-740) antibody
RASEF 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2D5 unconjugated
anti-RAS and EF Hand Domain Containing (RASEF) (AA 631-740) antibodyRASEF 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E3 unconjugated
anti-RAS and EF Hand Domain Containing (RASEF) antibodyRASEF 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB2745 unconjugated Recombinant Antibody
anti-RAS and EF Hand Domain Containing (RASEF) antibodyRASEF 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB5065 unconjugated Recombinant Antibody
anti-RAS and EF Hand Domain Containing (RASEF) (C-Term) antibodyRASEF 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-RAS and EF Hand Domain Containing (RASEF) (AA 194-243) antibodyRASEF 适用: 人, Cow, 犬, 马, 兔, Bat WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the RASEF antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the RASEF antibody at -20oC.
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- RASEF (RAS and EF Hand Domain Containing (RASEF))
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别名
- RASEF
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背景
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RASEF antibody detects Ras and EF hand domain containing protein, also known as RAB45, encoded by the RASEF gene. RASEF is a member of the Rab family of small GTP binding proteins with unique EF hand calcium binding motifs. This combination of domains links small GTPase activity with calcium regulated signaling, positioning RASEF as a multifunctional regulator of vesicular trafficking, cytoskeletal dynamics, and cell signaling. RASEF localizes to endosomal and cytoplasmic membranes, where it contributes to transport and signaling events.
RASEF antibody is widely applied in cancer research, cell biology, and immunology. RASEF has been identified as a tumor suppressor gene in uveal melanoma, where mutations or promoter hypermethylation reduce its expression. Conversely, altered RASEF activity has been observed in lung and breast cancer, suggesting context dependent functions. By detecting RASEF, researchers can investigate its dual roles in tumor biology and cell signaling.
Western blot assays detect RASEF protein in diverse tissue lysates. Immunohistochemistry maps expression in eye, lung, and immune tissues, while immunofluorescence highlights subcellular localization to endosomes and membranes. These methods provide robust tools for analyzing RASEF biology.
RASEF contributes to immune signaling through regulation of endosomal trafficking and receptor recycling. It may influence T cell activation, antigen presentation, and cytokine signaling by modulating endosomal compartments. Its calcium binding EF hand motifs suggest integration of calcium signaling with vesicle dynamics. By applying RASEF antibody, scientists can examine how small GTPases coordinate trafficking and signaling across biological systems.
Beyond immunity and cancer, RASEF has roles in ocular biology, where it is expressed in retinal tissues and linked to eye disease. It also contributes to cellular homeostasis through regulation of vesicle fusion and cytoskeletal remodeling. These diverse functions make RASEF antibody valuable for both basic science and translational research.
RASEF antibody from NSJ Bioreagents provides dependable specificity for detecting Ras and EF hand domain containing protein. Its performance across applications ensures accurate results in cancer biology, cell signaling, and immune regulation studies. -
UniProt
- Q8IZ41
抗原
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