Recombinant PRPF4 抗体
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北京 101111
Quick Overview for Recombinant PRPF4 抗体 (ABIN7882102)
抗原
See all PRPF4 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- PRPF4 Antibody / Pre mRNA processing factor 4
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纯化方法
- Affinity-chromatography
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免疫原
- A synthesized peptide derived from human PRPF4 was used as the immunogen for the PRPF4 antibody.
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亚型
- IgG
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anti-PRP4 Pre-mRNA Processing Factor 4 Homolog (PRPF4) (AA 1-522) antibody
PRPF4 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-PRP4 Pre-mRNA Processing Factor 4 Homolog (PRPF4) antibodyKD Validated PRPF4 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB975 unconjugated Recombinant Antibody
anti-PRP4 Pre-mRNA Processing Factor 4 Homolog (PRPF4) (AA 1-260) antibodyPRPF4 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-PRP4 Pre-mRNA Processing Factor 4 Homolog (PRPF4) antibodyPRPF4 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB980 unconjugated Recombinant Antibody
anti-PRP4 Pre-mRNA Processing Factor 4 Homolog (PRPF4) (AA 71-120) antibodyPRPF4 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼, 小鸡, Drosophila melanogaster, Hamster, 猴, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the PRPF4 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the PRPF4 antibody at -20oC.
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- PRPF4 (PRP4 Pre-mRNA Processing Factor 4 Homolog (PRPF4))
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别名
- PRPF4
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背景
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PRPF4 antibody recognizes pre mRNA processing factor 4, a nuclear protein essential for spliceosome assembly and pre mRNA splicing. PRPF4 is a component of the U4 and U6 small nuclear ribonucleoproteins and is required for the formation of the U4 or U6 U5 tri small nuclear ribonucleoprotein complex. This complex is indispensable for pre mRNA splicing, a fundamental process that ensures accurate removal of introns and joining of exons to produce mature messenger RNA.
PRPF4 antibody is highly relevant for studies of gene expression regulation and RNA biology. By detecting PRPF4, researchers can investigate how spliceosome components assemble and how splicing is regulated under different physiological conditions. Disruption of PRPF4 function impairs RNA processing and leads to widespread changes in gene expression, highlighting its importance in cell biology.
The antibody is suitable for western blotting, immunohistochemistry, immunofluorescence, and other molecular biology applications. In western blot assays, PRPF4 antibody recognizes nuclear protein bands, confirming expression across tissue and cell samples. Immunohistochemistry provides tissue localization, often highlighting nuclear distribution in actively transcribing cells. Immunofluorescence further resolves subnuclear localization and colocalization with other spliceosomal proteins.
Mutations in PRPF4 are linked to retinitis pigmentosa, a degenerative retinal disorder characterized by progressive vision loss. This connection underscores the physiological importance of splicing factors beyond general RNA metabolism. PRPF4 antibody therefore provides a valuable reagent for studies of inherited retinal disease as well as broader investigations of splicing related disorders.
Splicing dysregulation is also increasingly recognized in cancer and neurodegeneration. Aberrant splicing contributes to oncogenic transformation, resistance to therapy, and progression of neurodegenerative diseases. Detecting PRPF4 expression with specific antibodies enables researchers to connect splicing factor function with disease mechanisms and to evaluate therapeutic interventions targeting the spliceosome.
PRPF4 antibody from NSJ Bioreagents offers strong performance across assay types, ensuring accurate detection of this critical spliceosomal protein. It is a powerful tool for molecular biology, disease research, and therapeutic development focused on RNA regulation. -
UniProt
- O43172
抗原
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