Recombinant PKC 抗体
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Quick Overview for Recombinant PKC 抗体 (ABIN7882075)
抗原
See all PKC (PKC pan) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- PKC Antibody / Protein kinase C
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纯化方法
- Affinity-chromatography
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免疫原
- A synthesized peptide derived from human PKC was used as the immunogen for the PKC antibody.
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亚型
- IgG
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anti-Protein Kinase C (pan) (PKC pan) (pThr497) antibody
PKC pan 适用: 人, 大鼠, 小鼠 WB, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C (pan) (PKC pan) (AA 623-672) antibodyPKC pan 适用: 人, 大鼠, 小鼠 WB, IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C (pan) (PKC pan) (pSer660) antibodyPKC pan 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB5450 unconjugated Recombinant Antibody
anti-Protein Kinase C (pan) (PKC pan) (Thr497) antibodyPKC pan 适用: 人, 大鼠, 小鼠 WB, IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C (pan) (PKC pan) (pThr497) antibodyPKC pan 适用: 人, 大鼠, 小鼠 WB, IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the PKC antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the PKC antibody at -20oC.
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- PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))
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别名
- PKC
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背景
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PKC antibody detects Protein kinase C (PKC), a family of serine/threonine kinases that play critical roles in cellular signal transduction, gene expression, and membrane receptor regulation. The UniProt recommended name is Protein kinase C. This enzyme family mediates diverse biological responses by phosphorylating target proteins involved in proliferation, apoptosis, differentiation, and cytoskeletal organization. PKC enzymes are key downstream effectors of diacylglycerol (DAG) and calcium signaling pathways, serving as central hubs that integrate extracellular signals into intracellular responses.
Protein kinase C includes multiple isoforms that can be broadly divided into three classes: conventional (alpha, beta, gamma), novel (delta, epsilon, eta, theta), and atypical (zeta, iota/lambda). Each isoform differs in its activation requirements and tissue distribution. Conventional isoforms require both calcium and DAG for activation, whereas novel isoforms are calcium-independent but DAG-dependent. Atypical isoforms do not require either calcium or DAG, responding instead to other lipid mediators. These differences allow PKC family members to finely tune signaling cascades in a context-dependent manner.
Functionally, PKC regulates numerous processes such as receptor desensitization, ion channel modulation, transcription factor activation, and cytoskeletal remodeling. It is activated by various stimuli including growth factors, hormones, and neurotransmitters through phospholipase C-mediated generation of DAG. Once activated, PKC translocates to cellular membranes where it phosphorylates specific substrates, leading to altered protein activity and gene expression. In neurons, PKC modulates synaptic plasticity, while in cardiac and smooth muscle it influences contraction and metabolism.
The PKC gene family is expressed across nearly all tissues, with particular enrichment in brain, heart, and skeletal muscle. Dysregulation of PKC activity has been linked to pathological conditions such as cancer, diabetes, neurodegeneration, and cardiovascular disease. In oncology, PKC can act as either a tumor promoter or suppressor depending on the isoform and cellular context. Therefore, antibodies targeting PKC are valuable tools for investigating its expression patterns, activation states, and downstream signaling pathways.
PKC antibody is useful in immunohistochemistry, immunofluorescence, and related assays to assess protein localization, phosphorylation-dependent signaling, and isoform-specific expression. By detecting total PKC or specific subtypes, researchers can study its role in signal transduction and disease progression. NSJ Bioreagents provides PKC antibody reagents optimized for research in kinase signaling, cellular communication, and phosphoregulation studies. -
UniProt
- P05129
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途径
- Positive Regulation of Response to DNA Damage Stimulus
抗原
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