Recombinant NFE2 抗体
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Quick Overview for Recombinant NFE2 抗体 (ABIN7881833)
抗原
See all NFE2 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- NFE2 Antibody / Nuclear factor erythroid 2
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纯化方法
- Affinity chromatography
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免疫原
- A synthesized peptide derived from human NFE2 was used as the immunogen for the NFE2 antibody.
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亚型
- IgG
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anti-Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2) (AA 1-373) antibody
NFE2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C6 unconjugated
anti-Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2) antibodyNFE2 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB520 unconjugated Recombinant Antibody
anti-Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2) (Center) antibodyNFE2 适用: 人, 小鼠 WB, IF, IP, ICC, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2) (AA 108-157) antibodyNFE2 适用: 人, 马, 犬, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2) (N-Term) antibodyNFE2 适用: 人, 小鼠, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2) (AA 312-340), (C-Term) antibodyNFE2 适用: 人 WB 宿主: 兔 Polyclonal RB31295 unconjugated
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应用备注
- Optimal dilution of the NFE2 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the NFE2 antibody at -20oC.
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- NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))
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别名
- NFE2
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背景
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NFE2 antibody targets Nuclear factor erythroid 2, a transcription factor encoded by the NFE2 gene. NFE2 belongs to the basic leucine zipper family and is composed of a heterodimer of a large DNA-binding subunit (p45) and a small subunit from the Maf protein family. This transcription factor plays a central role in the regulation of erythroid and megakaryocytic cell differentiation. NFE2 antibody provides an essential reagent for studying gene expression programs controlling hematopoiesis, oxidative stress responses, and platelet production.
The p45 subunit of NFE2 binds to antioxidant response elements and regulates transcription of genes involved in heme biosynthesis, hemoglobin assembly, and detoxification of reactive oxygen species. In megakaryocytes, NFE2 is required for proplatelet formation and platelet release. Detection of this factor using NFE2 antibody helps identify regulatory pathways that maintain normal blood development and function.
Mutations or dysregulation of NFE2 are implicated in hematological disorders, including myeloproliferative neoplasms. Overexpression of NFE2 has been reported in polycythemia vera and essential thrombocythemia, linking the transcription factor to abnormal erythrocyte and platelet production. Research employing NFE2 antibody has revealed roles in leukemogenesis and abnormal megakaryopoiesis. Because of these connections, NFE2 is studied as a potential therapeutic target in blood cancers.
NFE2 antibody can be applied in western blotting, immunohistochemistry, and electrophoretic mobility shift assays. Western blotting confirms protein expression in erythroid cell lines, while immunohistochemistry highlights tissue-specific nuclear localization. In gene regulation studies, chromatin immunoprecipitation with NFE2 antibody allows mapping of target promoters controlled by this transcription factor.
NFE2 is also relevant in oxidative stress research. Its regulation of antioxidant genes links it to cellular defense mechanisms against free radicals and environmental toxins. Researchers using NFE2 antibody can explore how transcriptional control intersects with oxidative signaling and stress adaptation. NSJ Bioreagents supports hematology and transcription factor research by providing validated antibodies against NFE2. -
UniProt
- Q16621
抗原
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