Recombinant NDUFB4 抗体
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北京 101111
Quick Overview for Recombinant NDUFB4 抗体 (ABIN7881759)
抗原
See all NDUFB4 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- NDUFB4 Antibody / NADH dehydrogenase ubiquinone 1 beta subcomplex subunit 4
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纯化方法
- Affinity chromatography
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免疫原
- A synthesized peptide derived from human NDUFB4 was used as the immunogen for the NDUFB4 antibody.
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亚型
- IgG
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anti-NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 4, 15kDa (NDUFB4) (AA 1-90) antibody
NDUFB4 适用: 人 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 4, 15kDa (NDUFB4) (AA 1-129) antibodyNDUFB4 适用: 人, 大鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 4, 15kDa (NDUFB4) antibodyNDUFB4 适用: 人 WB, ICC 宿主: 兔 Monoclonal 24GB500 unconjugated Recombinant Antibody
anti-NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 4, 15kDa (NDUFB4) (AA 71-120) antibodyNDUFB4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 4, 15kDa (NDUFB4) antibodyNDUFB4 适用: 人, 大鼠, 小鼠 WB, IF, IHC, ICC 宿主: 兔 Monoclonal 30N45 unconjugated
anti-NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 4, 15kDa (NDUFB4) (C-Term) antibodyNDUFB4 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, Pig WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the NDUFB4 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the NDUFB4 antibody at -20oC.
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- NDUFB4 (NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 4, 15kDa (NDUFB4))
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别名
- NDUFB4
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背景
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NDUFB4 antibody detects NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, encoded by the NDUFB4 gene. This protein is a non catalytic accessory subunit of mitochondrial Complex I, the first enzyme complex of the oxidative phosphorylation system. Complex I catalyzes the transfer of electrons from NADH to ubiquinone, coupled with proton translocation across the inner mitochondrial membrane. NDUFB4 antibody provides researchers with an essential reagent for studying mitochondrial respiratory function, bioenergetics, and disease mechanisms.
Although NDUFB4 does not directly participate in electron transfer, it contributes to the structural stability and assembly of Complex I. Research using NDUFB4 antibody has shown that accessory subunits help maintain the integrity of the complex, ensuring efficient electron flow and proton pumping. Loss or dysfunction of accessory proteins like NDUFB4 destabilizes Complex I, leading to reduced oxidative phosphorylation and ATP production. This highlights the importance of even non catalytic subunits in respiratory chain function.
Mutations and defects in Complex I subunits, including NDUFB4, are associated with mitochondrial diseases such as Leigh syndrome, encephalopathy, and mitochondrial myopathy. Research using NDUFB4 antibody has demonstrated that absence or reduction of NDUFB4 impairs assembly of Complex I, leading to decreased activity and energy deficiency. Such dysfunctions contribute to neuromuscular symptoms, developmental delays, and metabolic acidosis. Because mitochondrial disease mechanisms are complex, NDUFB4 antibody serves as an important tool for dissecting assembly defects and functional outcomes.
In cancer, altered expression of Complex I subunits including NDUFB4 has been observed. Tumor cells often exhibit metabolic reprogramming, favoring glycolysis over oxidative phosphorylation. However, subsets of tumors rely on intact mitochondrial respiration, making Complex I subunits potential biomarkers and therapeutic targets. Studies with NDUFB4 antibody have shown correlations between expression levels and tumor progression, underscoring its relevance in oncology. Beyond cancer, mitochondrial dysfunction involving NDUFB4 contributes to neurodegenerative diseases, metabolic disorders, and aging related decline.
NDUFB4 antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms protein abundance in mitochondria rich tissues such as brain, heart, and muscle. Immunohistochemistry highlights tissue distribution, while immunofluorescence demonstrates mitochondrial localization using colocalization with markers such as COX IV. These approaches ensure that NDUFB4 antibody is useful in both clinical and basic mitochondrial research.
By supplying validated NDUFB4 antibody reagents, NSJ Bioreagents supports research into oxidative phosphorylation, mitochondrial disease, and cancer biology. Detection of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 enables researchers to explore how Complex I subunits maintain cellular energy production and contribute to disease when impaired. -
UniProt
- O95168
抗原
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