Recombinant NSDHL 抗体
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北京 101111
Quick Overview for Recombinant NSDHL 抗体 (ABIN7881756)
抗原
See all NSDHL 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- NSDHL Antibody / NADP-dependent steroid dehydrogenase-like protein
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纯化方法
- Affinity chromatography
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免疫原
- A synthesized peptide derived from human NSDHL was used as the immunogen for the NSDHL antibody.
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亚型
- IgG
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anti-NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) antibody
KD Validated NSDHL 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB510 unconjugated Recombinant Antibody
anti-NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) antibodyKD Validated NSDHL 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB485 unconjugated Recombinant Antibody
anti-NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) (AA 1-110) antibodyNSDHL 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6E3 unconjugated
anti-NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) (AA 1-240) antibodyKO Validated NSDHL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) (AA 1-373) antibodyNSDHL 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) (AA 154-211) antibodyNSDHL 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) (AA 1-373) antibodyNSDHL 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) (AA 181-230) antibodyNSDHL 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, Pig, 兔, 斑马鱼, Bat, 小鸡, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the NSDHL antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the NSDHL antibody at -20oC.
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- NSDHL (NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))
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别名
- NSDHL
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背景
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NSDHL antibody is designed to detect NAD(P)-dependent steroid dehydrogenase-like protein, an essential enzyme of the cholesterol biosynthetic pathway. NSDHL is localized to the endoplasmic reticulum membrane and functions as a C-4 sterol dehydrogenase, catalyzing the removal of methyl groups from sterol intermediates. This enzymatic activity is necessary for producing cholesterol and other sterols that are critical for membrane structure, signaling, and developmental processes.
NSDHL antibody is widely used in studies of metabolism, developmental biology, and genetic disorders. Mutations in the NSDHL gene are associated with CHILD syndrome (congenital hemidysplasia with ichthyosiform erythroderma and limb defects), an X-linked dominant disorder characterized by skin abnormalities and limb malformations. Detecting NSDHL with specific antibodies enables researchers to evaluate expression and function in disease models and clinical studies.
Applications of NSDHL antibody include western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, NSDHL antibody detects the protein with high specificity, confirming its expression across cell and tissue samples. Immunohistochemistry reveals distribution patterns in tissues involved in sterol metabolism, while immunofluorescence highlights its endoplasmic reticulum localization in cultured cells. These experimental approaches provide important insights into cholesterol biosynthesis at the molecular level.
NSDHL plays a vital role in cholesterol metabolism, and dysregulation of its activity has broader implications for human health. Abnormal sterol accumulation contributes to metabolic disease, cardiovascular dysfunction, and neurodevelopmental abnormalities. Using NSDHL antibody, researchers can track enzyme activity and explore how disruptions in cholesterol biosynthesis impact cellular and systemic function.
Beyond inherited disorders, NSDHL has been implicated in cancer biology. Cholesterol metabolism supports rapid tumor growth, and enzymes in the biosynthetic pathway are increasingly recognized as potential therapeutic targets. Monitoring NSDHL expression with antibodies allows investigators to connect sterol metabolism with oncogenic processes, highlighting new avenues for intervention.
NSDHL antibody from NSJ Bioreagents is a reliable reagent for studying sterol metabolism, developmental genetics, and cholesterol-related disease. Its specificity ensures accurate detection in multiple research settings, supporting basic and translational studies alike. -
UniProt
- Q15738
抗原
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