Myogenin 抗体
Our Local Distributor
北京 101111
Quick Overview for Myogenin 抗体 (ABIN7881725)
抗原
See all Myogenin (MYOG) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Anti-Myogenin Antibody
-
纯化方法
- Protein G affinity chromatography
-
免疫原
- Human recombinant protein was used as the immunogen for this anti-Myogenin antibody.
-
亚型
- IgG1, kappa
-
-
-
-
应用备注
-
The optimal dilution of the anti-Myogenin antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store the anti-Myogenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
-
-
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
-
别名
- Myogenin
-
背景
- Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.
-
UniProt
- P15173
-
途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
抗原
-