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Myogenin 抗体

The 小鼠 单克隆 anti-Myogenin antibody (Clone SPM144) (ABIN7881725) specifically detects Myogenin in IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN7881725
发货至: 中国
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Quick Overview for Myogenin 抗体 (ABIN7881725)

抗原

See all Myogenin (MYOG) 抗体
Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Myogenin antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

SPM144
  • 原理

    Anti-Myogenin Antibody

    纯化方法

    Protein G affinity chromatography

    免疫原

    Human recombinant protein was used as the immunogen for this anti-Myogenin antibody.

    亚型

    IgG1, kappa
  • 应用备注

    The optimal dilution of the anti-Myogenin antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store the anti-Myogenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • 抗原

    Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

    别名

    Myogenin

    背景

    Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.

    UniProt

    P15173

    途径

    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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