Keratin Basic 抗体
Our Local Distributor
北京 101111
Quick Overview for Keratin Basic 抗体 (ABIN7881441)
抗原
See all Keratin Basic 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Basic Cytokeratin Antibody
-
纯化方法
- Protein G affinity chromatography
-
免疫原
- Solubilized keratin extract from human stratum corneum was used as the immunogen for the Basic Cytokeratin antibody.
-
亚型
- IgG1, kappa
-
-
anti-Keratin Basic antibody
Carrier-free 适用: 人, 大鼠 IHC (p) 宿主: 小鼠 Monoclonal KRTH-1076 unconjugated
anti-Keratin Basic antibody适用: 人, 大鼠, 小鼠 WB, IF, IHC, FACS 宿主: 小鼠 Monoclonal SPM591 unconjugated
anti-Keratin Basic antibody适用: 人 IHC (p) 宿主: 兔 Monoclonal KRTH-1576R unconjugated Recombinant Antibody
anti-Keratin Basic antibodyCarrier-free 适用: 人 IHC (p) 宿主: 兔 Monoclonal KRTH-1576R unconjugated Recombinant Antibody
-
-
应用备注
-
Optimal dilution of the Basic Cytokeratin antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store the Basic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
-
-
- Keratin Basic
-
别名
- Basic Cytokeratin
-
背景
- This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This mAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
-
UniProt
- Q01546
抗原
-
