ICAM-3/CD50 抗体
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北京 101111
Quick Overview for ICAM-3/CD50 抗体 (ABIN7881232)
抗原
See all ICAM-3/CD50 (ICAM3) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-ICAM-3 Antibody
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纯化方法
- Protein G affinity chromatography
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免疫原
- Stimulated human leukocytes were used as the immunogen for the anti-ICAM-3 antibody.
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亚型
- IgG2b, kappa
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应用备注
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Optimal dilution of the anti-ICAM-3 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the anti-ICAM-3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
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别名
- ICAM-3
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背景
- Recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb is excellent for staining of formalin/paraffin tissues.
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UniProt
- P32942
抗原
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