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ICAM-3/CD50 抗体

The 小鼠 单克隆 anti-ICAM-3/CD50 antibody (Clone SPM505) (ABIN7881232) specifically detects ICAM-3/CD50 in IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN7881232
发货至: 中国
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Quick Overview for ICAM-3/CD50 抗体 (ABIN7881232)

抗原

See all ICAM-3/CD50 (ICAM3) 抗体
ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This ICAM-3/CD50 antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

SPM505
  • 原理

    Anti-ICAM-3 Antibody

    纯化方法

    Protein G affinity chromatography

    免疫原

    Stimulated human leukocytes were used as the immunogen for the anti-ICAM-3 antibody.

    亚型

    IgG2b, kappa
  • 应用备注

    Optimal dilution of the anti-ICAM-3 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store the anti-ICAM-3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • 抗原

    ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

    别名

    ICAM-3

    背景

    Recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb is excellent for staining of formalin/paraffin tissues.

    UniProt

    P32942
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