ABO 抗体
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北京 101111
Quick Overview for ABO 抗体 (ABIN7881056)
抗原
See all ABO 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- ABO Antibody / Blood Group Antigen H Type 2
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纯化方法
- Protein A/G affinity
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免疫原
- Human breast cancer MCF-7 cells were used as the immunogen for the ABO antibody.
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亚型
- IgM, kappa
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anti-Histo-blood group A transferase (ABO) (AA 54-354) antibody
ABO 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) (AA 273-354) antibodyABO 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 1B7 unconjugated
anti-Histo-blood group A transferase (ABO) (N-Term) antibodyABO 适用: 人, 小鼠, 大鼠 ELISA, IHC, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) (AA 41-68), (N-Term) antibodyABO 适用: 人 WB 宿主: 小鼠 Monoclonal 593CT3-2-2 unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人 IF 宿主: 小鼠 Monoclonal HEB-20 unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB3610 unconjugated Recombinant Antibody
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人, 小鼠, 大鼠 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人 IHC, IF 宿主: 小鼠 Monoclonal HE-193 unconjugated
anti-Histo-blood group A transferase (ABO) (AA 235-354) antibodyABO 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the ABO antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot the ABO antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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- ABO (Histo-blood group A transferase (ABO))
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别名
- ABO
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背景
- Recognizes the blood group H type 2 antigens, trisaccharide Fuc1 of human origin. The specificity of this MAb has been identified by haemagglutination tests, immunohistochemistry, binding inhibition studies, and absorption experiments performed with synthetic oligosaccharides. The antibody is virtually nonreactive with H type 1 antigen or with closely related type 2 structures (e.g., Y antigen). A46-B/B10 strongly agglutinates human erythrocytes according to the amount of H substance expressed and can, therefore, easily discriminate between blood groups A1 and A2 as well as A1B and A2B (A1 and A1B are not or only weakly agglutinated). In immunohistochemistry, this antibody seems to provide a highly specific reagent for a restricted number of carcinomas and epithelial lineages in tissue sections and in vitro. This was tested on 753 blood samples. It strongly agglutinates human erythrocytes of blood groups 0, A2 and A2B, but not or only very weakly A1 and A1B. This MAb promises to be useful in the determination of A and AB subgroups in blood group serology. This MAb might also be able to define B subgroups. Among 263 B blood samples, 45 (17.1 % ) were negative or only weakly positive with A46-B/B10.
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UniProt
- P16442
抗原
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