Golgi Marker 抗体 (CF®700)
The CF®700-conjugated 小鼠 单克隆 anti-Golgi Marker antibody (Clone GLG1-970) (ABIN7880920) specifically detects Golgi Marker in FACS 和 IF.
The antibody is reactive with 人 samples.
产品编号 ABIN7880920
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for Golgi Marker 抗体 (CF®700) (ABIN7880920)
抗原
Golgi Marker
适用
人
宿主
小鼠
克隆类型
单克隆
标记
CF®700
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF)
克隆位点
GLG1-970
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原理
- Golgi Marker Antibody
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纯化方法
- Protein G affinity chromatography
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免疫原
- The Golgi fraction from human liver cells was used as the immunogen for the Golgi Marker antibody.
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亚型
- IgG1, kappa
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anti-Golgi Marker antibody
Carrier-free 适用: 人 IHC (p) 宿主: 小鼠 Monoclonal GLG1-970 unconjugated
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应用备注
- Optimal dilution of the Golgi Marker antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 500 µL at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store the Golgi Marker antibody at 2-8oC, protected from light.
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- Golgi Marker
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背景
- This MAb recognizes a protein of 134 kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. Highest levels are expressed in pancreas, skeletal muscle, placenta, heart, testis and ovary. It is also found in the kidney, liver, lung and brain. It is expressed in both adult and fetal tissues. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.
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UniProt
- Q92896
抗原
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