Recombinant GGH 抗体
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北京 101111
Quick Overview for Recombinant GGH 抗体 (ABIN7880852)
抗原
See all GGH 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- GGH Antibody / Gamma glutamyl hydrolase
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纯化方法
- Affinity-chromatography
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免疫原
- A synthesized peptide derived from human Gamma glutamyl hydrolase was used as the immunogen for the GGH antibody.
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亚型
- IgG
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anti-gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH) (AA 229-256), (C-Term) antibody
GGH 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB31980 unconjugated
anti-gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH) (AA 7-34), (N-Term) antibodyGGH 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB20066 unconjugated
anti-gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH) (AA 14-42), (N-Term) antibodyGGH 适用: 人 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH) (AA 236-264), (C-Term) antibodyGGH 适用: 人 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH) (AA 25-318) antibodyGGH 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C3 unconjugated
anti-gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH) (C-Term) antibodyGGH 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH) (AA 219-293) antibodyGGH 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH) (AA 22-342) antibodyGGH 适用: Soybean WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH) (AA 25-230) antibodyGGH 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH) (AA 25-318) antibodyGGH 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the GGH antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the GGH antibody at -20oC.
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- GGH (gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH))
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别名
- GGH
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背景
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GGH antibody detects gamma glutamyl hydrolase, an enzyme encoded by the GGH gene. GGH hydrolyzes polyglutamylated folates and antifolate drugs, converting them into monoglutamyl forms that can be transported across membranes. This activity regulates intracellular folate homeostasis and influences the efficacy of antifolate chemotherapeutics such as methotrexate. By controlling folate metabolism, GGH contributes to nucleotide synthesis, DNA replication, and cell proliferation.
GGH antibody is widely applied in oncology, pharmacology, and metabolism research. Elevated GGH expression reduces the retention and potency of antifolate drugs, contributing to chemoresistance in leukemia and solid tumors. Conversely, altered GGH activity affects folate availability and one carbon metabolism, impacting DNA synthesis and repair. By detecting GGH, researchers can study how folate metabolism intersects with cancer biology, drug resistance, and metabolic disease.
Western blot assays detect GGH protein bands in liver and tumor extracts. Immunohistochemistry maps expression in proliferative tissues and tumors, while immunofluorescence highlights cytoplasmic localization in cells actively synthesizing DNA. These approaches provide insight into how GGH distribution correlates with metabolic activity and drug response.
GGH also plays roles in normal physiology, regulating folate supply for rapidly dividing cells in bone marrow, intestinal epithelium, and developing tissues. Dysregulation of GGH has been associated with megaloblastic anemia, cancer progression, and sensitivity to antifolate therapy. By applying GGH antibody, scientists can explore the balance between folate metabolism and proliferation in both health and disease.
GGH antibody from NSJ Bioreagents provides reliable specificity for studying gamma glutamyl hydrolase function. Its strong performance across multiple assays ensures accurate detection of this enzyme in basic research and translational studies of chemotherapy resistance. -
UniProt
- Q92820
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途径
- SARS-CoV-2 Protein Interactome
抗原
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