Recombinant FPGS 抗体
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北京 101111
Quick Overview for Recombinant FPGS 抗体 (ABIN7880807)
抗原
See all FPGS 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- FPGS Antibody / Folylpolyglutamate synthase
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纯化方法
- Affinity chromatography
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免疫原
- A synthesized peptide derived from human FPGS was used as the immunogen for the FPGS antibody.
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亚型
- IgG
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anti-Folylpolyglutamate Synthase (FPGS) (AA 210-439) antibody
FPGS 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Folylpolyglutamate Synthase (FPGS) (AA 304-330) antibodyFPGS 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21571 unconjugated
anti-Folylpolyglutamate Synthase (FPGS) (AA 135-232) antibodyFPGS 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Folylpolyglutamate Synthase (FPGS) (AA 443-546) antibodyFPGS 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Folylpolyglutamate Synthase (FPGS) (AA 265-314) antibodyFPGS 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Folylpolyglutamate Synthase (FPGS) antibodyFPGS 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Folylpolyglutamate Synthase (FPGS) antibodyFPGS 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the FPGS antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the FPGS antibody at -20oC.
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- FPGS (Folylpolyglutamate Synthase (FPGS))
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别名
- FPGS
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背景
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FPGS antibody detects Folylpolyglutamate synthase, encoded by the FPGS gene. Folylpolyglutamate synthase is a key enzyme in folate metabolism that catalyzes the addition of glutamate residues to folate and antifolate compounds, converting them into polyglutamated forms that are retained within cells. These polyglutamates are essential for nucleotide biosynthesis and methylation reactions, as they serve as cofactors in one-carbon metabolism. FPGS antibody provides researchers with a powerful tool to study folate biology, cancer metabolism, and drug resistance.
Folylpolyglutamate synthase is localized to both mitochondria and cytosol, ensuring polyglutamation occurs in multiple compartments. This enzyme plays an indispensable role in maintaining pools of tetrahydrofolate derivatives required for DNA synthesis and repair. Research using FPGS antibody has revealed that reduced enzyme activity decreases intracellular folate retention and disrupts nucleotide biosynthesis, leading to impaired proliferation. These findings explain why mutations or dysregulation of FPGS contribute to megaloblastic anemia and developmental defects.
In oncology, FPGS is highly relevant because it modulates the activity of antifolate chemotherapeutics such as methotrexate and pemetrexed. These drugs require polyglutamation by FPGS for retention and enhanced potency. Research using FPGS antibody has demonstrated that low expression of Folylpolyglutamate synthase confers resistance to antifolate therapy, while high activity sensitizes cells to treatment. This makes FPGS an important biomarker for predicting drug response in leukemia, lymphoma, and solid tumors. Studies continue to evaluate whether modulation of FPGS expression can overcome resistance and improve outcomes in antifolate-based regimens.
Beyond cancer, folate metabolism plays critical roles in pregnancy, neural tube development, and cardiovascular health. Research with FPGS antibody has shown that adequate polyglutamate production is required for normal development, and deficiencies in this pathway contribute to congenital malformations and disease. As such, FPGS remains an important protein in both clinical and nutritional studies. Immunohistochemistry and western blotting using FPGS antibody have confirmed its widespread expression across tissues, with particular enrichment in proliferative and metabolically active cells.
FPGS antibody is used in western blotting, immunohistochemistry, and enzyme activity assays. Western blotting measures relative abundance in normal and tumor tissue, immunohistochemistry demonstrates tissue distribution, and enzymatic assays link FPGS expression with catalytic activity. Together, these methods provide a full picture of how Folylpolyglutamate synthase supports cell metabolism, proliferation, and therapeutic responses.
By providing validated FPGS antibody reagents, NSJ Bioreagents supports studies of folate biology, drug resistance, and one-carbon metabolism. Detection of Folylpolyglutamate synthase enables researchers to explore fundamental metabolic pathways and their implications in health and disease. -
UniProt
- Q05932
抗原
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